关键词： Forensic validation studies Multiplex PCR assay Reference databasing STR STRtyper-32G kit

Mesh ： Amelogenin / genetics Asians / ethnology genetics Databases, Nucleic Acid Humans INDEL Mutation / genetics Microsatellite Repeats / genetics Multiplex Polymerase Chain Reaction / instrumentation Nucleic Acid Amplification Techniques / methods Sensitivity and Specificity Species Specificity

来  源：   DOI：10.1016/j.forsciint.2021.110977   PDF(Sci-hub)

Abstract：
The STRtyper-32G PCR Amplification Kit is a 6-dye multiplex system that combines the 30 autosomal STR loci with an Indel site (YIndel) and the sex-determinant locus Amelogenin. In addition to more loci, Master Mix has been optimized to amplify DNA on different substrates. The autosomal STR loci contained in this novel system meet the compatibility of requirements for databasing. In this study, the developmental validation study of the STRtyper-32G Kit followed the guidelines of SWGDAM (Scientific Working Group on DNA Analysis Methods), including PCR-based studies, species specificity, inhibitors, sensitivity, precision, repeatability, stutter, DNA mixtures, concordance studies, and population genetics studies. The validation results indicate that the new multiplex system is a robust tool for forensic database applications.
摘要：
STRtyper-32GPCR扩增试剂盒是一种6染料多重系统，将30个常染色体STR基因座与Indel位点（YIndel）和性别决定基因座Amelogenin结合在一起。除了更多的基因座，已优化主混合物以在不同底物上扩增DNA。该新型系统中包含的常染色体STR基因座满足数据库化要求的兼容性。在这项研究中,STRtyper-32GKit的开发验证研究遵循了SWGDAM（DNA分析方法科学工作组）的指南，包括基于PCR的研究，物种特异性，抑制剂，灵敏度,精度,重复性,口吃,DNA混合物,一致性研究，和群体遗传学研究。验证结果表明，新的多路复用系统是取证数据库应用的可靠工具。
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