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Abstract：
The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has infected millions of people worldwide. SARS-CoV-2 belongs to the Betacoronavirus genus, containing the mouse hepatitis virus (MHV), an extensively studied animal coronavirus. Since MHV and SARS-CoV-2 share the same genus, MHV could offer insights relative to SARS-CoV-2 studies. MHV-3 strain causes hepatitis and cellular injury, making MHV-3 infection one of the best models for this debilitating disease. Surrogate coronaviruses have been used for virus resistance and inactivation studies, and although real-life conditions using SARS-CoV-2 should be encouraged, their use needs to be balanced with safety and costs. MHV can be manipulated under BSL2 laboratory conditions, unlike SARS-CoV-2, making it a model for studying the virucidal effects on coronaviruses. In this study, we used the betacoronavirus MHV-3 as a model to investigate the virucidal activity of an air disinfection equipment named STR Solution®, an air sterilizer with patented technology. MHV-3 was dried on different surfaces and exposed at varying distances from the STR Solution® equipment and at different exposure times. The residual infectivity was evaluated using the endpoint method. There was not a significant reduction (mean p-value = 0.4) of the viral titer under STR Solution® exposition. STR Solution® caused a slight decrease of the infectious particles\' titer (> 1 log10) only under the following conditions: polypropylene at 3 m, for 1 and 3 h (1.2 log10 reduction TCID50) and Sus domesticus skin at 0.05 m, for 1 h (1.3 log10 reduction TCID50), and at 3 m for 1 h (1.2 log10 reduction TCID50). These and other studies confirm the usefulness of this model to evaluate virucidal activity.
摘要：
严重急性呼吸道综合症冠状病毒-2（SARS-CoV-2）已感染全球数百万人。SARS-CoV-2属于Betacoronavirus属，含有小鼠肝炎病毒（MHV），一种广泛研究的动物冠状病毒。由于MHV和SARS-CoV-2具有相同的属，MHV可以提供与SARS-CoV-2研究相关的见解。MHV-3菌株导致肝炎和细胞损伤，使MHV-3感染成为这种使人衰弱的疾病的最佳模型之一。替代冠状病毒已用于病毒抗性和灭活研究，尽管应该鼓励使用SARS-CoV-2的现实生活条件，它们的使用需要与安全性和成本相平衡。MHV可以在BSL2实验室条件下操作，与SARS-CoV-2不同，使其成为研究对冠状病毒的杀病毒作用的模型。在这项研究中,我们使用β冠状病毒MHV-3作为模型来研究名为STRSolution®的空气消毒设备的杀病毒活性，具有专利技术的空气消毒器。MHV-3在不同的表面上干燥,并以与STRSolution®设备不同的距离和不同的暴露时间暴露。使用终点方法评估残余感染性。在STR溶液®暴露下,病毒滴度没有显著降低(平均p值=0.4)。STRSolution®仅在以下条件下引起感染性颗粒滴度的轻微下降(>1log10):3米聚丙烯,对于1和3小时（1.2log10减少TCID50）和0.05m处的Susdomesticus皮肤，持续1小时(1.3log10减少TCID50)，并在3米下持续1小时(1.2log10减少TCID50)。这些和其他研究证实了该模型对评估杀病毒活性的有用性。
