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Abstract：
Metanil yellow is a food dye that has harmful impacts on different body systems. Scutellarin has antioxidant, antiapoptotic, and anti-inflammatory activities. The aim of the current research was to study the effect of chronic administration of metanil yellow on the cerebellar cortex of rats and to evaluate the protective effect of scutellarin. Forty adult male rats were allocated into four groups: group I acted as control, group II was administrated scutellarin (100 mg/kg/day), group III was administrated metanil yellow (200 mg/kg/day), and group IV was administrated scutellarin and metanil yellow as in group II and group III. The agents were administered via oral gavage for 8 weeks. Metanil yellow induced a significant rise in the malondialdehyde coupled with a significant reduction in the superoxide dismutase and glutathione peroxidase. The Purkinje cells were irregular and shrunken with condensed nuclei. A significant elevation in glial fibrillary acidic protein (GFAP) and cleaved caspase-3 as well as a significant reduction of synaptophysin expression were revealed in comparison with the control group. Interestingly, few changes were noticed in rats given metanil yellow concomitant with scutellarin. In conclusion, scutellarin could protect against metanil yellow-induced alterations in the cerebellar cortex by reducing oxidative stress and minimizing gliosis.
摘要：
Metanil黄是一种对不同身体系统有有害影响的食品染料。scutellarin有抗氧化剂，抗凋亡，和抗炎活性。本研究的目的是研究长期服用甲尼黄对大鼠小脑皮质的影响，并评估灯盏乙素的保护作用。将40只成年雄性大鼠分为四组：I组作为对照组，II组给予灯盏乙素（100mg/kg/天），第III组服用甲硝胺黄（200mg/kg/天），第IV组和第II组和第III组分别给予灯盏乙素和米他尼黄。通过口服管饲法施用药剂8周。Metanil黄诱导丙二醛的显着升高，同时超氧化物歧化酶和谷胱甘肽过氧化物酶的显着降低。Purkinje细胞不规则且收缩，核浓缩。与对照组相比，神经胶质原纤维酸性蛋白（GFAP）和裂解的caspase-3显着升高，突触素表达显着降低。有趣的是,在给予甲氨酚黄和灯盏乙素的大鼠中几乎没有发现变化。总之,灯盏乙素可以通过减少氧化应激和最大程度地减少神经胶质增生来防止甲胺黄引起的小脑皮质改变。
