PDF(Pubmed)
Abstract：
Chronic graft-versus-host disease (cGvHD) is a systemic alloimmune and autoimmune disorder and a major late complication of allogeneic hematopoietic stem cell transplantation (alloHSCT). The disease is characterized by an altered homeostasis of the humoral immune response. Immunoglobulin G (IgG) glycoprotein is the main effector molecule of the humoral immune response. Changes in IgG glycosylation are associated with a number of autoimmune diseases. IgG glycosylation analysis was done by the means of liquid chromatography in the National Institutes of Health (NIH) cohort of 213 cGvHD patients. The results showed statistically significant differences with regards to cGvHD NIH joint/fascia and skin score, disease activity and intensity of systemic immunosuppression. ROC analysis confirmed that IgG glycosylation increases specificity and sensitivity of models using laboratory parameters and markers of inflammation associated with cGvHD (eosinophil count, complement components C3 and C4 and inflammation markers: albumin, CRP and thrombocyte count). This research shows that IgG glycosylation may play a significant role in cGvHD pathology. Further research could contribute to the understanding of the disease biology and lead to the clinical biomarker development to allow personalized approaches to chronic GvHD therapy.
摘要：
慢性移植物抗宿主病（cGvHD）是一种全身性同种免疫和自身免疫性疾病，是异基因造血干细胞移植（alloHSCT）的主要晚期并发症。该疾病的特征在于体液免疫应答的稳态改变。免疫球蛋白G(IgG)糖蛋白是体液免疫应答的主要效应分子。IgG糖基化的变化与许多自身免疫性疾病有关。在美国国立卫生研究院(NIH)的213名cGvHD患者的队列中通过液相色谱法进行IgG糖基化分析。结果显示，关于cGvHDNIH关节/筋膜和皮肤评分，疾病活动和全身免疫抑制的强度。ROC分析证实，使用实验室参数和与cGvHD相关的炎症标志物（嗜酸性粒细胞计数，补体成分C3和C4以及炎症标志物：白蛋白，CRP和血小板计数）。这项研究表明，IgG糖基化可能在cGvHD病理中起重要作用。进一步的研究可能有助于对疾病生物学的理解，并导致临床生物标志物的开发，以允许个性化的方法来治疗慢性GvHD。
