PDF(Pubmed)
Abstract：
UNASSIGNED: Essential oils found frequently in plants are well known for their activities against bacteria, viruses, and fungi, and antioxidant properties. This study aimed to analyze egg yolk replacement by seed oils of Gossypium spp. (cotton), Balanites aegyptiaca (desert date), and Sesamum indicum (sesame) in semen extender, on ram sperm quality chilled at 4°C and frozen-thawed.
UNASSIGNED: Ejaculates were collected from adult rams and refrigerated at 4°C in a Tris-based extender containing 1.25%, 2.5%, 5%, and 10% of Gossypium spp., B. aegyptiaca, and S. indicum seed oils, to evaluate which were the two best extenders for comparison with BIOXcell, a commercial extender for deep freezing ram semen.
UNASSIGNED: The data showed that sperm movements analyzed by the CASA system were faster in extenders supplemented with 2.5-5% of cottonseed oil and 1.25-10% of sesame oil, whereas in the extender containing B. aegyptiaca oil, all seminal parameters studied had the worst values. During the sperm-freezing process, 5% of cottonseed oil and 5% sesame seed oil were selected from the first study, with sesame oil reaching the best sperm quality. Thus, sperm motility and velocity were 44.14±13.99%, 24.44±12.6%, and 25.92±11.50%; and 20.26±9.56%, 8.76±6.38%, and 9.42±5.40%, respectively, for sesame oil, cottonseed oil, and BIOXcell.
UNASSIGNED: In summary, 2.5-10% of cottonseed oil and 1.25-10% of sesame seed oil can replace egg yolk in a Tris-egg yolk-based extender. Moreover, a Tris-based extender supplemented with 5% sesame seed oil could be an alternative for deep freezing ram semen, even though these results need to be confirmed with semen collected from rams with appropriate sexual rest.
UNASSIGNED: Ejaculates were collected from adult rams and refrigerated at 4°C in a Tris-based extender containing 1.25%, 2.5%, 5%, and 10% of Gossypium spp., B. aegyptiaca, and S. indicum seed oils, to evaluate which were the two best extenders for comparison with BIOXcell, a commercial extender for deep freezing ram semen.
UNASSIGNED: The data showed that sperm movements analyzed by the CASA system were faster in extenders supplemented with 2.5-5% of cottonseed oil and 1.25-10% of sesame oil, whereas in the extender containing B. aegyptiaca oil, all seminal parameters studied had the worst values. During the sperm-freezing process, 5% of cottonseed oil and 5% sesame seed oil were selected from the first study, with sesame oil reaching the best sperm quality. Thus, sperm motility and velocity were 44.14±13.99%, 24.44±12.6%, and 25.92±11.50%; and 20.26±9.56%, 8.76±6.38%, and 9.42±5.40%, respectively, for sesame oil, cottonseed oil, and BIOXcell.
UNASSIGNED: In summary, 2.5-10% of cottonseed oil and 1.25-10% of sesame seed oil can replace egg yolk in a Tris-egg yolk-based extender. Moreover, a Tris-based extender supplemented with 5% sesame seed oil could be an alternative for deep freezing ram semen, even though these results need to be confirmed with semen collected from rams with appropriate sexual rest.
摘要：
■在植物中经常发现的精油以其对抗细菌的活性而闻名。病毒,和真菌，和抗氧化性能。本研究旨在分析棉籽油对蛋黄的替代作用。（棉），埃及Balanites埃及（沙漠日期），和芝麻（芝麻）在精液补充剂中，在4°C下冷冻并冻融的公羊精子质量。
■从成年公羊中收集射精，并在含有1.25％的Tris基补充剂中于4°C下冷藏，2.5%,5%,和10％的棉属物种。,B.埃及埃及，和S.in种子油，为了评估与BIOXcell比较的两个最佳扩展器，一种用于深冻RAM精液的商业延伸剂。
■数据显示，通过CASA系统分析的精子运动在补充了2.5-5％的棉籽油和1.25-10％的芝麻油的补充剂中更快，而在含有埃及芽孢杆菌油的补充剂中，所有研究的开创性参数都有最差的值.在精子冷冻过程中,从第一个研究中选择了5％的棉籽油和5％的芝麻油，用芝麻油达到最好的精子质量。因此，精子活力和速度为44.14±13.99%，24.44±12.6%，25.92±11.50%；20.26±9.56%，8.76±6.38%，和9.42±5.40%，分别,芝麻油，棉籽油,BIOXcell
■总之，2.5-10％的棉籽油和1.25-10％的芝麻油可以替代Tris蛋黄基补充剂中的蛋黄。此外，补充有5％芝麻油的Tris-based补充剂可能是深层冷冻精液的替代品，即使这些结果需要通过从具有适当性休息的公羊身上收集的精液来证实。
■从成年公羊中收集射精，并在含有1.25％的Tris基补充剂中于4°C下冷藏，2.5%,5%,和10％的棉属物种。,B.埃及埃及，和S.in种子油，为了评估与BIOXcell比较的两个最佳扩展器，一种用于深冻RAM精液的商业延伸剂。
■数据显示，通过CASA系统分析的精子运动在补充了2.5-5％的棉籽油和1.25-10％的芝麻油的补充剂中更快，而在含有埃及芽孢杆菌油的补充剂中，所有研究的开创性参数都有最差的值.在精子冷冻过程中,从第一个研究中选择了5％的棉籽油和5％的芝麻油，用芝麻油达到最好的精子质量。因此，精子活力和速度为44.14±13.99%，24.44±12.6%，25.92±11.50%；20.26±9.56%，8.76±6.38%，和9.42±5.40%，分别,芝麻油，棉籽油,BIOXcell
■总之，2.5-10％的棉籽油和1.25-10％的芝麻油可以替代Tris蛋黄基补充剂中的蛋黄。此外，补充有5％芝麻油的Tris-based补充剂可能是深层冷冻精液的替代品，即使这些结果需要通过从具有适当性休息的公羊身上收集的精液来证实。
