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Abstract：
Liver fluke, Fascioloides magna, is an important non-native parasite introduced to Europe, posing a threat to survival of local wildlife populations. The aim of this study was to assess the serum and liver protein profile of control and F. magna infected wild boars, by means of shotgun tandem mass tag - based quantitative high resolution proteomics approach. In serum, 4 differentially abundant proteins were found out of total 1073 identified, while in liver from 3520 identified proteins, 116 were differentially abundant between healthy and F. magna infected wild boars. Pathway analysis revealed that most of the proteins differing in abundance are involved in metabolism, biological oxidations, cellular responses to stimuli, fatty acid metabolism, and others. Validation of proteomic results was performed for paraoxonase-1, ceruloplasmin, glutathione S-transferase and liver enzymes by ELISA and automated assays. Complementary analysis of liver and serum in F. magna infection enabled insight into changes of proteome profile of the host at local and sistemic level. Our findings showed that chronic infection with F. magna is associated with immune response in host, oxidative stress and metabolomic changes in liver. SIGNIFICANCE: Liver fluke infections are recognised as worldwide neglected diseases with considerable veterinary and public health importance. Pathological changes, clinical signs and outcome of F. magna infection are strongly related to the type of final hosts and their different tolerance to infection. In order to gain insight into host-parasite interactions in wild boars, dead-end host for F. magna, we assessed proteomics profile of serum and liver of control animals and those infected with F. magna. Proteomics analysis of serum and liver in parallel showed as advantageous and beneficial, demonstrating protein alterations mainly at local level. Bioinformatics analysis enabled elucidation of molecular pathways associated with F. magna infection. Identification and validation of proteins associated with infection may have added value to current tools for efficient liver fluke control.
摘要：
肝吸虫,麦格纳龙,是引入欧洲的一种重要的非本地寄生虫，对当地野生动物种群的生存构成威胁。这项研究的目的是评估对照和F.magna感染的野猪的血清和肝脏蛋白谱，借助基于鸟枪串联质量标签的定量高分辨率蛋白质组学方法。在血清中，在总共1073种蛋白质中发现了4种差异丰富的蛋白质，而在肝脏中从3520个鉴定出的蛋白质，健康野猪和麦格纳感染野猪之间有116种差异丰富。路径分析显示，大多数丰度不同的蛋白质都与代谢有关，生物氧化，细胞对刺激的反应，脂肪酸代谢，和其他人。对对氧磷酶-1、铜蓝蛋白、谷胱甘肽S-转移酶和肝酶通过ELISA和自动测定。对F.magna感染中的肝脏和血清的补充分析使人们能够了解宿主在局部和持续水平上的蛋白质组变化。我们的发现表明，用F.magna慢性感染与宿主的免疫反应有关，肝脏氧化应激和代谢组学变化。意义：肝吸虫感染被认为是世界范围内被忽视的疾病，具有相当大的兽医和公共卫生重要性。病理变化,F.magna感染的临床体征和结果与最终宿主的类型及其对感染的不同耐受性密切相关。为了深入了解野猪中寄主-寄生虫的相互作用，麦格纳的死胡同主机，我们评估了对照动物和感染F.magna的动物的血清和肝脏的蛋白质组学特征。血清和肝脏的蛋白质组学平行分析显示是有利的和有益的，主要在局部水平显示蛋白质改变。生物信息学分析能够阐明与麦格纳感染相关的分子途径。与感染相关的蛋白质的鉴定和验证可能为有效控制肝吸虫的当前工具增加了价值。
