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Abstract：
The aim of the study was to quantify the micro-architectural changes of the jaw bone in response to ovariectomy, exposed or not to bisphosphonate treatment. A total of 47 Wistar rats were ovariectomized (OVX) or sham-operated (shOVX) and exposed to osteoporosis preventive treatment for eight weeks either with bisphosphonates (alendronate, ALN; group OVX-ALN) three days/week at a dose of 2 mg/kg or with saline solution (untreated control condition; group OVX). The bone morphometric parameters of the trabecular jaw bone were assessed using ex vivo micro-computed tomography. The regions of interest investigated in the maxilla were the inter-radicular septum of the second molar and the tuber. The regions quantified in the mandible included the three molar regions and the condyle. A one-way analysis of variance followed by pairwise comparison using Tukey\'s HSD and the Games-Howell test was conducted to explore significant differences between the groups. In the maxilla, OVX decreased the bone volume in the inter-radicular septum of the second molar. Bisphosphonate treatment was able to prevent this deterioration of the jaw bone. The other investigated maxillary regions were not affected by (un)treated ovariectomy. In the mandible, OVX had a significant negative impact on the jaw bone in the buccal region of the first molar and the inter-radicular region of the third molar. Treatment with ALN was able to prevent this jaw bone loss. At the condyle site, OVX significantly deteriorated the trabecular connectivity and shape, whereas preventive bisphosphonate treatment showed a positive effect on this trabecular bone region. No significant results between the groups were observed for the remaining regions of interest. In summary, our results showed that the effects of ovariectomy-induced osteoporosis are manifested at selected jaw bone regions and that bisphosphonate treatment is capable to prevent these oral bone changes.
摘要：
该研究的目的是量化卵巢切除术后颌骨的微观结构变化，暴露或不暴露于双膦酸盐治疗。总共对47只Wistar大鼠进行了卵巢切除（OVX）或假手术（shOVX），并用双膦酸盐（阿仑膦酸盐，ALN；OVX-ALN组）每周三天，剂量为2mg/kg或使用盐溶液（未处理的对照条件；OVX组）。使用离体显微计算机断层扫描评估小梁颌骨的骨形态参数。在上颌骨中研究的感兴趣区域是第二磨牙的神经根间隔和块茎。下颌骨中量化的区域包括三个磨牙区域和髁。进行了单向方差分析，然后使用Tukey的HSD和Games-Howell检验进行了成对比较，以探索组间的显着差异。在上颌骨,OVX减少了第二磨牙的神经根间隔的骨体积。双膦酸盐治疗能够防止颌骨的这种恶化。其他研究的上颌区域不受（未）治疗的卵巢切除术的影响。在下颌骨中，OVX对第一磨牙颊区和第三磨牙根间区的颌骨有显著的负面影响。用ALN治疗能够防止这种颌骨丢失。在髁部位，OVX显著恶化了小梁的连通性和形状，而预防性双膦酸盐治疗对该骨小梁区域显示出积极作用。对于剩余的感兴趣区域,在组间没有观察到显著结果。总之,我们的研究结果表明，卵巢切除术引起的骨质疏松症的影响表现在选定的颌骨区域，双膦酸盐治疗能够预防这些口腔骨变化。
