关键词: agranular leukocyte ductus deferens halo cell mesenchymal cell monitor lizard reptiles

Mesh : Animals Epididymis Lizards Male Microscopy, Electron, Transmission Spermatozoa Vas Deferens / ultrastructure

来  源:   DOI:10.1017/S1431927621012046   PDF(Sci-hub)

Abstract:
The ductus deferens is a fundamental part of the male genital tract and the continuation of the epididymal duct. As a male secondary sex organ, the ductus deferens plays a crucial role in the nourishment, storage, and maturation of spermatozoa. Some studies have provided information about the ductus deferens structure in reptiles; however, the full description of the ductus deferens remains to be clarified. The current study aimed to describe the Nile monitor lizard (Varanus niloticus) ductus deferens from histological, histochemical, and ultrastructural perspectives. The results revealed that the ductus deferens is formed histologically from two main cell types: principal and basal. The principal cells were tall and filled with periodic acid Schiff (+)/alcian blue (−) cytoplasmic granules. The basal cells were found just above the basement membrane. By transmission electron microscopy, the principal cells exhibited typical protein-secreting cell features. Additionally, some intraepithelial cells, such as halo cells, undifferentiated mesenchymal cells, and agranular leukocytes, were identified. This study presents the first detailed description of the Varanus niloticus ductus deferens. Further immunohistochemical studies are required to explore the function(s) of the cellular components.
摘要:
输精管是男性生殖道的基本组成部分,也是附睾管的延续。作为男性第二性器官,输精管在营养中起着至关重要的作用,storage,精子的成熟.一些研究提供了有关爬行动物输精管结构的信息;但是,输精管的完整描述仍有待澄清。当前的研究旨在从组织学上描述尼罗河监测蜥蜴(Varanusniloticus)输精管,组织化学,和超微结构的观点。结果表明,输精管在组织学上由两种主要细胞类型形成:主要细胞和基底细胞。主要细胞很高,充满了高碘酸Schiff()/alcian蓝(-)细胞质颗粒。基底细胞位于基底膜正上方。通过透射电子显微镜,主要细胞表现出典型的蛋白质分泌细胞特征。此外,一些上皮细胞,如光环细胞,未分化的间充质细胞,和中性白细胞,已确定。本研究首次对尼罗氏输精管进行了详细描述。需要进一步的免疫组织化学研究来探索细胞组分的功能。
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