PDF(Pubmed)
Abstract：
Organoids have revolutionized cancer research as highly adaptable models that enable an array of experimental techniques to interrogate tissue morphology and function. Because they preserve the genetic, phenotypic, and behavioral traits of their source tissue, organoids have gained traction as the most relevant models for drug discovery, tracking therapeutic response and for personalized medicine. As organoids are indisputably becoming a mainstay of cancer research, this review specifically addresses how colon-derived organoids can be perfected as multidimensional, scalable, reproducible models of healthy, pre-neoplastic and neoplastic conditions of the colon and for use in high-throughput \"Phase-0\" human clinical trials-in-a-dish.
摘要：
类器官作为高度适应性的模型彻底改变了癌症研究，使一系列实验技术能够询问组织形态和功能。因为它们保留了基因，表型，以及它们来源组织的行为特征，类器官作为药物发现最相关的模型获得了吸引力，跟踪治疗反应和个性化医疗。随着类器官无可争议地成为癌症研究的支柱，这篇综述特别讨论了如何将结肠衍生的类器官完善为多维，可扩展，可复制的健康模型，结肠的肿瘤前和肿瘤条件，用于高通量的“0期”人体临床试验。
