Abstract：
Very little research has investigated the effects of ultraendurance exercise on the bioenergetic status of muscle. The primary objective of this case study was to characterize the changes that occur in skeletal muscle mitochondria in response to a 100-km ultramarathon in monozygotic twins. A second objective was to determine whether mitochondrial function is altered by consuming a periodized low-carbohydrate, high-fat diet during training compared with a high-carbohydrate diet.
One pair of male monozygotic twins ran 100 km on treadmills after 4 wk of training on either a high-carbohydrate or periodized low-carbohydrate, high-fat diet. Muscle biopsies were collected 4 wk before the run, as well as 4 and 52 h postrun. Blood draws were also performed immediately before as well as 4 and 52 h after the run.
Four hours postrun, respiratory capacity, citrate synthase activity, and mitochondrial complex protein content were decreased. Two days later, both twins showed signs of rapid recovery in several of these measures. Furthermore, blood levels of creatine phosphokinase, C-reactive protein, and aspartate transaminase were elevated 4 h after the run but partially recovered 2 d later.
Although there were some differences between the twins, the primary finding is that there is significant mitochondrial impairment induced by running 100 km, which rapidly recovers within 2 d. These results provide ample rationale for future investigations of the effects of ultraendurance activity on mitochondrial function.
One pair of male monozygotic twins ran 100 km on treadmills after 4 wk of training on either a high-carbohydrate or periodized low-carbohydrate, high-fat diet. Muscle biopsies were collected 4 wk before the run, as well as 4 and 52 h postrun. Blood draws were also performed immediately before as well as 4 and 52 h after the run.
Four hours postrun, respiratory capacity, citrate synthase activity, and mitochondrial complex protein content were decreased. Two days later, both twins showed signs of rapid recovery in several of these measures. Furthermore, blood levels of creatine phosphokinase, C-reactive protein, and aspartate transaminase were elevated 4 h after the run but partially recovered 2 d later.
Although there were some differences between the twins, the primary finding is that there is significant mitochondrial impairment induced by running 100 km, which rapidly recovers within 2 d. These results provide ample rationale for future investigations of the effects of ultraendurance activity on mitochondrial function.
摘要：
很少有研究研究超耐力运动对肌肉生物能状态的影响。本病例研究的主要目的是表征单卵双胞胎中100公里超马拉松反应中骨骼肌线粒体发生的变化。第二个目标是确定线粒体功能是否通过消耗周期化的低碳水化合物而改变，与高碳水化合物饮食相比，训练期间的高脂肪饮食。
一对男性单卵双胞胎经过4周的高碳水化合物或低碳水化合物训练后，在跑步机上跑了100公里，高脂肪饮食.在跑步前4周收集肌肉活检，以及4小时和52小时post。还在运行前立即以及运行后4和52小时进行抽血。
四小时后，呼吸能力,柠檬酸合酶活性，线粒体复合物蛋白含量降低。两天后,在这些措施中，这对双胞胎都显示出快速康复的迹象。此外，血肌酸磷酸激酶水平，C反应蛋白,天门冬氨酸转氨酶在运行后4小时升高，但在2天后部分恢复。
虽然这对双胞胎之间存在一些差异,主要发现是跑步100公里会引起明显的线粒体损伤，在2d内迅速恢复。这些结果为未来研究超耐力活性对线粒体功能的影响提供了充分的理由。
一对男性单卵双胞胎经过4周的高碳水化合物或低碳水化合物训练后，在跑步机上跑了100公里，高脂肪饮食.在跑步前4周收集肌肉活检，以及4小时和52小时post。还在运行前立即以及运行后4和52小时进行抽血。
四小时后，呼吸能力,柠檬酸合酶活性，线粒体复合物蛋白含量降低。两天后,在这些措施中，这对双胞胎都显示出快速康复的迹象。此外，血肌酸磷酸激酶水平，C反应蛋白,天门冬氨酸转氨酶在运行后4小时升高，但在2天后部分恢复。
虽然这对双胞胎之间存在一些差异,主要发现是跑步100公里会引起明显的线粒体损伤，在2d内迅速恢复。这些结果为未来研究超耐力活性对线粒体功能的影响提供了充分的理由。
