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Abstract：
Aberrant glycosylation has long been known to be associated with cancer, since it is involved in key mechanisms such as tumour onset, development and progression. This review will focus on protein glycosylation studies in cells, tissue, urine and serum in the context of prostate cancer. A dedicated section will cover the glycoforms of prostate specific antigen, the molecule that, despite some important limitations, is routinely tested for helping prostate cancer diagnosis. Our aim is to provide readers with an overview of mass spectrometry-based glycoproteomics of prostate cancer. From this perspective, the first part of this review will illustrate the main strategies for glycopeptide enrichment and mass spectrometric analysis. The molecular information obtained by glycoproteomic analysis performed by mass spectrometry has led to new insights into the mechanism linking aberrant glycosylation to cancer cell proliferation, migration and immunoescape.
摘要：
众所周知，糖基化异常与癌症有关，因为它涉及肿瘤发病等关键机制，发展和进步。这篇综述将集中在细胞中的蛋白质糖基化研究，组织,尿液和血清在前列腺癌的背景下。专门的部分将涵盖前列腺特异性抗原的糖型，分子，尽管有一些重要的限制，常规测试有助于前列腺癌的诊断。我们的目的是为读者提供基于质谱的前列腺癌糖蛋白质组学的概述。从这个角度来看,本综述的第一部分将说明糖肽富集和质谱分析的主要策略。通过质谱进行的糖蛋白质组学分析获得的分子信息导致了对将异常糖基化与癌细胞增殖联系起来的机制的新见解。迁移和免疫逃逸。
