PDF(Sci-hub)
Abstract：
Designing clinical applicable polymeric composite scaffolds for auricular cartilage tissue engineering requires appropriate mechanical strength and biological characteristics. In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the \"steel bars reinforced concrete\" structure in architecture and their chondrogenic functions were also investigated. We found that the Ap silk fiber-based scaffolds reinforced by PLLA PMs (MAF) exhibited superior physical properties (the mechanical properties in particular) as compared to the Bm silk fiber-based scaffolds reinforced by PLLA PMs (MBF). Furthermore, in vitro evaluation of chondrogenic potential showed that the MAF provided better cell adhesion, viability, proliferation and GAG secretion than the MBF. Therefore, the MAF are promising in auricular cartilage tissue engineering and relevant plastic surgery-related applications.
摘要：
设计用于耳软骨组织工程的临床适用的聚合物复合支架需要适当的机械强度和生物学特性。在这项研究中,基于丝纤维的支架与聚-L-乳酸多孔微球（PLLAPM）与Bombyxmori（Bm）或Antheraeapernyi（Ap）丝纤维结合在一起，受到“钢筋增强混凝土”结构的启发，并研究了它们的软骨功能。我们发现，与由PLLAPM（MBF）增强的Bm丝纤维基支架相比，由PLLAPM（MAF）增强的Ap丝纤维基支架表现出优异的物理性能（特别是机械性能）。此外，软骨形成潜能的体外评估表明，MAF提供了更好的细胞粘附，生存能力，增殖和GAG分泌比MBF。因此,MAF在耳软骨组织工程和相关整形外科相关应用中很有前途。
