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Abstract：
BACKGROUND: Cowpea is a crop widely used in developing countries due its rusticity. Besides its rich genotypic variability, most breeding programs do not explore its potential to improve elements uptake. Selenium (Se) is a scarce element in most soils, resulting in its deficiency being common in human diets. This study aimed to evaluate the interaction between biofortification with Se and genotypic variation in cowpea, on the concentrations of Se in roots, leaves + stem and grains.
METHODS: Twenty-nine cowpea genotypes were grown in a greenhouse in the absence (control) and presence of Se (12.5 μg Se kg-1 soil) as sodium selenate, in fully randomized scheme. The plants were cultivated until grains harvest. The following variables were determined: roots dry weight (g), leaves + stems dry weight (g), grains dry weight (g), Se concentration (mg kg-1) in roots, leaves + stems and grains, and Se partitioning to shoots and grains.
RESULTS: Selenium application increased the Se concentration in roots, leaves + stems and grains in all genotypes. At least twofold variation in grain Se concentration was observed among genotypes. Selenium application did not impair biomass accumulation, including grain dry weight. Genotype \"BRS Guariba\" had the largest Se concentration in grains and leaves + stems. Genotype MNC04-795 F-158 had the largest partitioning of Se to shoots and grain, due to elevated dry weights of leaves + stems and grain, and high Se concentrations in these tissues.
CONCLUSIONS: This information might be valuable in future breeding programs to select for genotypes with better abilities to accumulate Se in grain to reduce widespread human Se undernutrition.
METHODS: Twenty-nine cowpea genotypes were grown in a greenhouse in the absence (control) and presence of Se (12.5 μg Se kg-1 soil) as sodium selenate, in fully randomized scheme. The plants were cultivated until grains harvest. The following variables were determined: roots dry weight (g), leaves + stems dry weight (g), grains dry weight (g), Se concentration (mg kg-1) in roots, leaves + stems and grains, and Se partitioning to shoots and grains.
RESULTS: Selenium application increased the Se concentration in roots, leaves + stems and grains in all genotypes. At least twofold variation in grain Se concentration was observed among genotypes. Selenium application did not impair biomass accumulation, including grain dry weight. Genotype \"BRS Guariba\" had the largest Se concentration in grains and leaves + stems. Genotype MNC04-795 F-158 had the largest partitioning of Se to shoots and grain, due to elevated dry weights of leaves + stems and grain, and high Se concentrations in these tissues.
CONCLUSIONS: This information might be valuable in future breeding programs to select for genotypes with better abilities to accumulate Se in grain to reduce widespread human Se undernutrition.
摘要：
背景：Cw豆是一种由于其质朴而在发展中国家广泛使用的作物。除了其丰富的基因型变异性，大多数育种计划都没有探索其改善元素吸收的潜力。硒（Se）是大多数土壤中的稀缺元素，导致其缺乏在人类饮食中很常见。这项研究旨在评估生物强化与硒之间的相互作用和cow豆的基因型变异，根中硒的浓度，叶子+茎和谷物。
方法：在不存在（对照）和存在硒（12.5μgSekg-1土壤）的情况下，在温室中生长了29种cow豆基因型，在完全随机方案中。种植植物直到收获谷物。确定以下变量：根干重（g），叶+茎干重（g），谷物干重（g），根中硒浓度（mgkg-1），叶子+茎和谷物，硒分配到芽和谷物中。
结果：施硒增加了根中的硒浓度，所有基因型的叶子+茎和谷物。在基因型之间观察到谷物硒浓度至少有两倍的变化。硒的施用不会损害生物量的积累，包括谷物干重。基因型“BRSGuariba”在谷物和叶片茎中的硒浓度最大。基因型MNC04-795F-158对芽和谷物的硒分配最大，由于叶片+茎和谷物的干重升高，和高硒浓度在这些组织。
结论：这些信息可能在未来的育种计划中很有价值，可以选择具有更好的能力在谷物中积累硒以减少广泛的人类硒营养不足的基因型。
方法：在不存在（对照）和存在硒（12.5μgSekg-1土壤）的情况下，在温室中生长了29种cow豆基因型，在完全随机方案中。种植植物直到收获谷物。确定以下变量：根干重（g），叶+茎干重（g），谷物干重（g），根中硒浓度（mgkg-1），叶子+茎和谷物，硒分配到芽和谷物中。
结果：施硒增加了根中的硒浓度，所有基因型的叶子+茎和谷物。在基因型之间观察到谷物硒浓度至少有两倍的变化。硒的施用不会损害生物量的积累，包括谷物干重。基因型“BRSGuariba”在谷物和叶片茎中的硒浓度最大。基因型MNC04-795F-158对芽和谷物的硒分配最大，由于叶片+茎和谷物的干重升高，和高硒浓度在这些组织。
结论：这些信息可能在未来的育种计划中很有价值，可以选择具有更好的能力在谷物中积累硒以减少广泛的人类硒营养不足的基因型。
