PDF(Sci-hub)
Abstract：
Cadmium (Cd) is a ubiquitous environmental pollutant, which mainly input to the aquatic environment through discharge of industrial and agricultural waste, can be a threat to human and animal health. Selenium (Se) possesses a beneficial role in protecting animals and ameliorating the toxic effects of Cd. However, the comparative antagonistic effects of different Se sources such as inorganic, organic Se and nano-form Se on Cd toxicity are still under-investigated. Hence, the purpose of this study was to evaluate the comparative of Se sources antagonism on Cd-induced nephrotoxicity via oxidative stress and selenoproteome transcription. In the present study, Cd-diet disturbed in the system balance of 5 trace elements (Zinc (Zn), copper (Cu), Iron (Fe), Se, Cd) and impaired renal function. Se sources, including nano- Se (NS), Se- yeast (SY), sodium selenite (SS) and mixed selenium (MS) significantly recovered the balance of 4 trace elements (Zn, Cu, Cd, Se) and renal impaired indexes (blood urea nitrogen (BUN) and creatinine (CREA)). Histological appearance of Cd-treated kidney indicated renal tubular epithelial vacuoles, particle degeneration and enlarged capsular space. Ultrastructure observation results illustrated that Cd-induced mitochondrial cristae reduction, membrane disappearance, and nuclear deformation. Treatment with Se sources, NS appeared a better impact on improving kidney tissues against the pathological alterations resulting from Cd administration. Meanwhile, NS reflected a significant impact on relieving Cd-induced kidney oxidative damage, and significantly restored the antioxidant defense system of the body. Our findings also showed NS ameliorated the Cd-induced downtrends expression of selenoproteome and selenoprotein synthesis related transcription factors. Overall, NS was the most effective Se source in avoiding of Cd cumulative toxicity, improving antioxidant capacity and regulating of selenoproteome transcriptome and selenoprotein synthesis related transcription factors expression, which contributes to ameliorate Cd-induced nephrotoxicity in chickens. These results demonstrated diet supplement with NS may prove to be an effective approach for alleviating Cd toxicity and minimizing Cd -induced health risk.
摘要：
镉(Cd)是一种普遍存在的环境污染物,主要通过排放工业和农业废物进入水环境，会威胁到人类和动物的健康。硒（Se）在保护动物和改善Cd的毒性作用方面具有有益的作用。然而,不同硒源的比较拮抗作用，如无机，有机硒和纳米形式硒对Cd的毒性研究仍不足。因此，这项研究的目的是评估硒源拮抗镉通过氧化应激和硒蛋白质组转录诱导的肾毒性的比较。在本研究中,镉饮食在5种微量元素(锌(Zn)，铜(Cu),铁(Fe),Se,Cd）和肾功能受损。硒源,包括纳米Se(NS)，酵母（SY），亚硒酸钠（SS）和混合硒（MS）显着恢复了4种微量元素的平衡（Zn，Cu,Cd,硒）和肾损害指标（血尿素氮（BUN）和肌酐（CREA）。Cd处理的肾脏的组织学外观表明肾小管上皮空泡，颗粒变性和囊间隙扩大。超微结构观察结果表明，Cd诱导线粒体cr减少，膜消失，和核变形。用硒源处理，NS对改善肾脏组织抵抗Cd给药引起的病理改变具有更好的影响。同时,NS反映了对减轻Cd诱导的肾脏氧化损伤的显着影响，并显著恢复了身体的抗氧化防御系统。我们的发现还表明NS改善了Cd诱导的硒蛋白质组和硒蛋白合成相关转录因子的下降趋势表达。总的来说,NS是避免Cd累积毒性的最有效的硒源，提高抗氧化能力,调节硒蛋白质组转录组和硒蛋白合成相关转录因子的表达,这有助于改善镉诱导的鸡肾毒性。这些结果表明，饮食补充NS可能是减轻Cd毒性和减少Cd引起的健康风险的有效方法。
