PDF(Pubmed)
Abstract：
Molecular hydrogen has been suggested to have a cytoprotective effect on the whole body and to enhance exercise performances. However, the effect of hydrogen-rich gas mixture (HG) inhalation on physiological responses has been poorly investigated. We examined the impact of acute HG inhalation on subsequent oxidative stress, muscle damage, and exercise performances during the recovery period after a strenuous exercise. This is a two-trial, double-blind, crossover, repeated measures study. Eight physically active male volunteers inhaled HG (estimated fraction of inspired oxygen and hydrogen were 21.57 and 4.08% at most, respectively) or normal gas (placebo, ambient air 400 m above sea level) during a 60-minute recovery phase after oxidative stress-inducing exercise) completion comprising 30-minute treadmill running at an intensity corresponding to 75% of maximal oxygen uptake and squat jumps (5 sets × 10 repetitions). Before oxidative stress-inducing exercise and 10 minutes after the post-exercise gas inhalation, blood and urine samples were obtained and exercise performances (jumping ability; pedaling power output; muscle strength) were evaluated. Post-exercise HG inhalation attenuated the increase in urinary 8-hydroxydeoxyguanosine excretion rate (P < 0.05), a DNA oxidation marker, and the reduction in the countermovement jump height (P < 0.05), compared with Placebo inhalation. Other exercise performances and blood oxidative stress and muscle damage markers did not differ between HG and Placebo inhalation. Moreover, the increase in urinary 8-hydroxydeoxyguanosine excretion rate was significantly associated with countermovement jump performance reduction (r = -0.78, P < 0.01). These findings suggested that HG inhalation during post-exercise recovery period might improve exercise performance via reducing systemic oxidative damage. The study was approved by the Human Research Ethics Committee of the University of Yamanashi (approval No. H29-006) on June 28, 2017.
摘要：
分子氢已被认为对整个身体具有细胞保护作用并增强运动表现。然而,吸入富氢气体混合物（HG）对生理反应的影响研究甚少。我们检查了急性HG吸入对随后的氧化应激的影响，肌肉损伤,以及剧烈运动后恢复期的运动表现。这是两次审判,双盲,交叉，重复措施研究。八名身体活跃的男性志愿者吸入了HG（吸入的氧气和氢气的估计比例最高为21.57％和4.08％，分别)或正常气体(安慰剂，海拔400m以上的环境空气）在氧化应激诱导运动后的60分钟恢复阶段）完成，包括30分钟的跑步机以相当于最大摄氧量和深蹲跳跃的75％的强度运行（5套×10重复）。氧化应激诱导运动前和运动后气体吸入后10分钟，采集血液和尿液样本，并评估运动表现（跳跃能力；踩踏力输出；肌肉力量）。运动后吸入HG减弱了尿8-羟基脱氧鸟苷排泄率的增加(P<0.05),DNA氧化标记，和反运动跳跃高度的降低（P<0.05），与安慰剂吸入相比。HG和安慰剂吸入之间的其他运动表现以及血液氧化应激和肌肉损伤标志物没有差异。此外，尿8-羟基脱氧鸟苷排泄率的增加与反运动跳成绩降低显著相关(r=-0.78,P<0.01)。这些发现表明，运动后恢复期吸入HG可能通过减少全身氧化损伤来改善运动表现。该研究获得了山梨大学人类研究伦理委员会的批准（批准号：H29-006）于2017年6月28日。
