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Abstract：
In this study, a strain producing β-glucanase and protease, identified as Bacillus velezensis Y1, was isolated from the manure of piglet. We attempted to produce β-glucanase and protease after optimization of various process parameters with the submerged fermentation. The effects of each factor on producing β-glucanase and protease were as follows: temperature > time > pH > loaded liquid volume. The properties of the β-glucanase showed that the most suitable reaction temperature was 65 °C and pH was 6.0. However for protease optimum reaction temperature was 50 °C, and pH was 6.0. The amplified PCR fragments of β-glucanase and protease were 1434 bp containing an open reading frame of 1413 bp encoding a protein with 444 amino acids and 1752 bp containing an open reading frame of 1521 bp encoding a protein with 506 amino acids, respectively. So, the study demonstrated a viable approach of using newly identified B. velezensis Y1 strain for the maximum yield of two industrially important enzymes.
摘要：
在这项研究中,产生β-葡聚糖酶和蛋白酶的菌株，从仔猪粪便中分离出，鉴定为velezensis芽孢杆菌Y1。在通过深层发酵优化各种工艺参数后，我们尝试生产β-葡聚糖酶和蛋白酶。各因素对β-葡聚糖酶和蛋白酶产生的影响如下:温度>时间>pH>装液量。β-葡聚糖酶的性质表明，最合适的反应温度为65°C，pH为6.0。然而，对于蛋白酶的最佳反应温度为50°C，pH为6.0。扩增的β-葡聚糖酶和蛋白酶的PCR片段为1434bp，包含编码444个氨基酸的蛋白质的1413bp的开放阅读框和1752bp，包含编码506个氨基酸的蛋白质的1521bp的开放阅读框，分别。所以,该研究证明了使用新鉴定的B.velezensisY1菌株获得两种工业上重要的酶的最大产量的可行方法。
