%0 Journal Article
%T Production of β-glucanase and protease from Bacillus velezensis strain isolated from the manure of piglets.
%A Khalid A
%A Ye M
%A Wei C
%A Dai B
%A Yang R
%A Huang S
%A Wang Z
%J Prep Biochem Biotechnol
%V 51
%N 5
%D 2021
%M 33108947
%F 3.141
%R 10.1080/10826068.2020.1833344
%X In this study, a strain producing β-glucanase and protease, identified as Bacillus velezensis Y1, was isolated from the manure of piglet. We attempted to produce β-glucanase and protease after optimization of various process parameters with the submerged fermentation. The effects of each factor on producing β-glucanase and protease were as follows: temperature > time > pH > loaded liquid volume. The properties of the β-glucanase showed that the most suitable reaction temperature was 65 °C and pH was 6.0. However for protease optimum reaction temperature was 50 °C, and pH was 6.0. The amplified PCR fragments of β-glucanase and protease were 1434 bp containing an open reading frame of 1413 bp encoding a protein with 444 amino acids and 1752 bp containing an open reading frame of 1521 bp encoding a protein with 506 amino acids, respectively. So, the study demonstrated a viable approach of using newly identified B. velezensis Y1 strain for the maximum yield of two industrially important enzymes.