The type VI secretion system 2 of Vibrio parahaemolyticus is regulated by QsvR.

• 影响指数: 3.848
• 发表时间: Dec 2020
• 来源期刊: Microb Pathog
• DOI: 10.1016/j.micpath.2020.104579
• PMID: 33091577
• 文章类型: Journal Article
• 中科院分区: Q3
• 方向: 医学
• 作者列表: Qiu Y,Hu L,Yang W,Yin Z,Zhou D,Yang H,Zhang Y

关键词: QsvR; T6SS2; Vibrio parahaemolyticus

Mesh: Bacterial Proteins / genetics metabolism; Gene Expression Regulation, Bacterial; HeLa Cells; Humans; Type VI Secretion Systems / genetics; Vibrio parahaemolyticus / genetics metabolism

Abstract

The type VI secretion system 2 (T6SS2) gene locus of Vibrio parahaemolyticus is comprised of three operons, VPA1027-1024, VPA1043-1028, and VPA1044-1046. QsvR is a virulence regulator of V. parahaemolyticus. In this study, the regulation of VPA1027, VPA1043 and VPA1044 by QsvR was investigated by primer extension, quantitative real-time PCR, LacZ fusion, electrophoretic mobility shift assay and DNase I footprinting. The results demonstrated that QsvR binds to the promoter-proximal DNA regions of each of these three operons, activating their transcription. T6SS2 was shown to predominately contribute to V. parahaemolyticus adhesion, with qsvR deletion significantly decreasing V. parahaemolyticus adhesion to HeLa cells. Thus, QsvR is not only a positive regulator of T6SS2 gene transcription but also a mediator of V. parahaemolyticus adhesion to host cells.