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Abstract：
Circular RNAs (CircRNAs) are a type of non-coding RNAs, which contain a covalently closed loop structure without 5\' to 3\' free ends. CircRNAs play essential roles in the regeneration of secondary hair follicle (SHF) and cashmere growth in goats. CircRNA-1926 was previously identified in SHF of cashmere goats, but its potential roles are unclear. In this study, we confirmed the expression of circRNA-1926 in SHF bulge of nine cashmere goats with a significantly higher level at anagen than that of telogen. Through the use of both overexpression and siRNA interference, we showed that circRNA-1926 promoted the differentiation of SHF stem cell into hair follicle lineage in cashmere goats which was evaluated via indictor genes Keratin 7 and Keratin 17. Using RNA pull-down, we found that circRNA-1926 bound with miR-148a/b-3p. Additionally, our data indicated that circRNA-1926 promoted the expression of the CDK19 gene. Using dual-luciferase reporter assays, it was revealed that circRNA-1926 positively regulated the CDK19 expression through miR-148a/b-3p. The results from this study demonstrated that circRNA-1926 contributes the differentiation of SHF stem cells into hair follicle lineages in cashmere goats via sponging miR-148a/b-3p to enhance CDK19 expression.
摘要：
环状RNA（CircRNAs）是一种非编码RNA，其包含没有5'至3'自由端的共价闭环结构。CircRNAs在山羊次级毛囊(SHF)再生和羊绒生长中起重要作用。CircRNA-1926先前在绒山羊的SHF中鉴定出，但其潜在作用尚不清楚。在这项研究中,我们证实了circRNA-1926在9只绒山羊的SHF凸起中的表达，其在生长期的水平明显高于静止期。通过使用过表达和siRNA干扰，我们发现circRNA-1926促进了绒山羊SHF干细胞向毛囊谱系的分化，这通过指示基因角蛋白7和角蛋白17进行了评估。使用RNA下拉，我们发现circRNA-1926与miR-148a/b-3p结合。此外,我们的数据表明circRNA-1926促进了CDK19基因的表达.使用双荧光素酶报告基因测定，研究显示,circRNA-1926通过miR-148a/b-3p正调控CDK19的表达.这项研究的结果表明，circRNA-1926通过海绵miR-148a/b-3p增强CDK19表达，有助于绒山羊SHF干细胞分化为毛囊谱系。
