PDF(Sci-hub)
Abstract：
BACKGROUND: Non-cholera Vibrio bacteria are a major cause of foodborne illness in the United States. Raw oysters are commonly implicated in gastroenteritis caused by pathogenic Vibrio parahaemolyticus. In response to outbreaks in 1997-1998, the US Food and Drug Administration developed a nation-wide quantitative microbial risk assessment (QMRA) of V. parahaemolyticus in raw oysters in 2005. The QMRA identified information gaps that new research may address. Incidence of sporadic V. parahaemolyticus illness has recently increased and, as oyster consumption increases and sea temperatures rise, V. parahaemolyticus outbreaks may become more frequent, posing health concerns. Updated and region-specific QMRAs will improve the accuracy and precision of risk of infection estimates.
OBJECTIVE: We identify research to support an updated QMRA of V. parahaemolyticus from oysters harvested in Chesapeake Bay and Puget Sound, focusing on observational and experimental research on post-harvest practices (PHPs) published from 2004 to 2019.
METHODS: A predefined search strategy was applied to PubMed, Embase, Scopus, Science.gov, NAL Agricola, and Google Scholar. Study eligibility criteria were defined using a population, intervention, comparator, and outcome statement. Reviewers independently coded abstracts for inclusion/exclusion using predefined criteria. Data were extracted and study quality and relevance evaluated based on published guidance for food safety risk assessments. Findings were synthesized using a weight of evidence approach.
RESULTS: Of 12,174 articles retrieved, 93 were included for full-text review. Twenty-seven studies were found to be high quality and high relevance, including studies on cold storage, high hydrostatic pressure, depuration, and disinfectant, and other PHPs. High hydrostatic pressure consistently emerged as the most effective PHP in reducing abundance of V. parahaemolyticus.
CONCLUSIONS: Limitations of the knowledge base and review approach involve the type and quantity of data reported. Future research should focus on PHPs for which few or no high quality and high relevance studies exist, such as irradiation and relaying.
OBJECTIVE: We identify research to support an updated QMRA of V. parahaemolyticus from oysters harvested in Chesapeake Bay and Puget Sound, focusing on observational and experimental research on post-harvest practices (PHPs) published from 2004 to 2019.
METHODS: A predefined search strategy was applied to PubMed, Embase, Scopus, Science.gov, NAL Agricola, and Google Scholar. Study eligibility criteria were defined using a population, intervention, comparator, and outcome statement. Reviewers independently coded abstracts for inclusion/exclusion using predefined criteria. Data were extracted and study quality and relevance evaluated based on published guidance for food safety risk assessments. Findings were synthesized using a weight of evidence approach.
RESULTS: Of 12,174 articles retrieved, 93 were included for full-text review. Twenty-seven studies were found to be high quality and high relevance, including studies on cold storage, high hydrostatic pressure, depuration, and disinfectant, and other PHPs. High hydrostatic pressure consistently emerged as the most effective PHP in reducing abundance of V. parahaemolyticus.
CONCLUSIONS: Limitations of the knowledge base and review approach involve the type and quantity of data reported. Future research should focus on PHPs for which few or no high quality and high relevance studies exist, such as irradiation and relaying.
摘要：
背景：非霍乱弧菌是美国食源性疾病的主要原因。生牡蛎通常与致病性副溶血性弧菌引起的胃肠炎有关。为了应对1997-1998年的爆发，美国食品和药物管理局于2005年对生牡蛎中的副溶血性弧菌进行了全国性的定量微生物风险评估（QMRA）。QMRA确定了新研究可能解决的信息差距。散发性副溶血性弧菌病的发病率最近有所增加，随着牡蛎消费量的增加和海水温度的升高，五、副溶血病暴发可能会变得更加频繁，提出健康问题。更新和特定地区的QMRA将提高感染风险估计的准确性和准确性。
目的：我们确定了一项研究，以支持从切萨皮克湾和普吉特海湾收获的牡蛎中获得的副溶血性弧菌的最新QMRA，专注于2004年至2019年发表的收获后实践（PHP）的观察和实验研究。
方法：将预定义的搜索策略应用于PubMed，Embase,Scopus,Science.gov,NALAgricola,谷歌学者。研究合格标准是使用人群定义的，干预，比较器,和结果声明。审阅者使用预定义的标准独立编码摘要以包含/排除。根据已发布的食品安全风险评估指南，提取数据并评估研究质量和相关性。使用证据权重方法综合研究结果。
结果：在检索到的12,174篇文章中，93个被纳入全文审查。27项研究被发现是高质量和高相关性的，包括冷藏方面的研究,高静水压力，净化，和消毒剂，其他PHP。高静水压力始终是减少副溶血性弧菌丰度的最有效PHP。
结论：知识库和审查方法的局限性涉及报告的数据类型和数量。未来的研究应集中在很少或没有高质量和高相关性研究的PHP上，如辐照和中继。
目的：我们确定了一项研究，以支持从切萨皮克湾和普吉特海湾收获的牡蛎中获得的副溶血性弧菌的最新QMRA，专注于2004年至2019年发表的收获后实践（PHP）的观察和实验研究。
方法：将预定义的搜索策略应用于PubMed，Embase,Scopus,Science.gov,NALAgricola,谷歌学者。研究合格标准是使用人群定义的，干预，比较器,和结果声明。审阅者使用预定义的标准独立编码摘要以包含/排除。根据已发布的食品安全风险评估指南，提取数据并评估研究质量和相关性。使用证据权重方法综合研究结果。
结果：在检索到的12,174篇文章中，93个被纳入全文审查。27项研究被发现是高质量和高相关性的，包括冷藏方面的研究,高静水压力，净化，和消毒剂，其他PHP。高静水压力始终是减少副溶血性弧菌丰度的最有效PHP。
结论：知识库和审查方法的局限性涉及报告的数据类型和数量。未来的研究应集中在很少或没有高质量和高相关性研究的PHP上，如辐照和中继。
