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Abstract：
We aimed to explore the role of TLR4 (rs4986790) polymorphism in the nasopharyngeal (NP) bacterial colonization and its consequent impact on the development of childhood asthma. A semi-quantitative culture of NP swabs was performed on 473 children at 2 months of age and on 213 children at 13 months of age. TLR4 polymorphism was analyzed for 396 children. Children were followed from birth to the age of 7.5 years and the final outcome was physician-diagnosed asthma. The associations between TLR4 genotype, bacterial colonization, and asthma were analyzed. Children with TLR4 AG or GG genotype were more often colonized with Moraxella catarrhalis at 2 months of age (p = 0.009) and Haemophilus influenzae at 13 months of age (p = 0.018). Children who were colonized with H. influenzae at 13 months of age had a significantly higher risk of later development of asthma (p = 0.004). M. catarrhalis or H. Influenzae colonization at 2 months of age or TLR4 genotype Asp299Gly were not associated with the development of childhood asthma. TLR4 Asp299Gly polymorphism was associated with an increased risk of colonization of M. catarrhalis and H. influenzae in children. The colonization with H. influenzae at 13 months of age was associated with a higher risk of later development of childhood asthma.
摘要：
我们旨在探讨TLR4（rs4986790）多态性在鼻咽（NP）细菌定植中的作用及其对儿童哮喘发展的影响。对473名2个月大的儿童和213名13个月大的儿童进行了NP拭子的半定量培养。对396名儿童进行TLR4多态性分析。随访儿童从出生到7.5岁，最终结果是医生诊断的哮喘。TLR4基因型之间的关联，细菌定植，和哮喘进行了分析。具有TLR4AG或GG基因型的儿童在2个月大（p=0.009）和13个月大流感嗜血杆菌（p=0.018）时更容易定植。在13个月大的时候定植有流感嗜血杆菌的儿童有明显更高的以后发展为哮喘的风险(p=0.004)。2月龄时卡他莫拉菌或H.流感定植或TLR4基因型Asp299Gly与儿童哮喘的发展无关。TLR4Asp299Gly多态性与儿童粘膜炎莫拉菌和流感嗜血杆菌定植风险增加相关。流感嗜血杆菌在13月龄时的定植与儿童哮喘后期发展的较高风险相关。
