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Abstract：
The Colonic Mucosal Barrier (CMB) is the site of interaction between the human body and the colonic microbiota. The mucus is the outer part of the CMB and is considered as the front-line defense of the colon. It separates the host epithelial lining from the colonic content, and it has previously been linked to health and diseases. In this study, we assessed the relationship between red meat and whole-grain intake and (1) the thickness of the colonic mucus (2) the expression of the predominant mucin gene in the human colon (MUC2). Patients referred to colonoscopy at the University Hospital of Southern Denmark- Sonderjylland were enrolled between June 2017 and December 2018, and lifestyle data was collected in a cross-sectional study design. Colonic biopsies, blood, urine, and fecal samples were collected. The colonic mucus and bacteria were visualized by immunostaining and fluorescence in situ hybridization techniques. We found a thinner mucus was associated with high red meat intake. Similarly, the results suggested a thinner mucus was associated with high whole-grain intake, albeit to a lesser extent than red meat. This is the first study assessing the association between red meat and whole-grain intake and the colonic mucus in humans. This study is approved by the Danish Ethics Committee (S-20160124) and the Danish Data Protecting Agency (2008-58-035). A study protocol was registered at clinical trials.gov under NCT04235348.
摘要：
结肠粘膜屏障(CMB)是人体与结肠微生物群之间相互作用的位点。粘液是CMB的外部，被认为是结肠的前线防御。它将宿主上皮衬里与结肠内容物分开，它以前与健康和疾病有关。在这项研究中,我们评估了红肉与全谷摄入量之间的关系，以及（1）结肠粘液的厚度（2）人结肠中主要粘蛋白基因（MUC2）的表达。2017年6月至2018年12月，在南丹麦大学医院接受结肠镜检查的患者入选，生活方式数据是在横断面研究设计中收集的。结肠活检,血,尿液,并收集粪便样本。通过免疫染色和荧光原位杂交技术可视化结肠粘液和细菌。我们发现较薄的粘液与红肉摄入量高有关。同样，结果表明，较薄的粘液与全麦摄入量高有关，尽管程度比红肉小。这是第一项评估红肉和全谷物摄入量与人类结肠粘液之间关联的研究。本研究得到丹麦伦理委员会(S-20160124)和丹麦数据保护机构(2008-58-035)的批准。根据NCT04235348，一项研究方案已在clinicaltrials.gov注册。
