背景:粪便免疫化学测试(FIT)是筛查结直肠癌的一种廉价且方便的方式。然而,其检测结直肠癌和癌症前体的一次性敏感性有限.对使用FIT残留缓冲液的非血红蛋白含量来增强结肠瘤形成检测越来越感兴趣。
目的:从文献中建立一个框架,对FIT残留缓冲液中的候选生物标志物进行分类,用于非侵入性大肠癌筛查。
方法:搜索策略评估了PubMed,Scopus,WebofScience,Embase,和谷歌学者的出版物到2023年10月25日,搜索词包括FIT,缓冲区,OC传感器,生物标志物,微生物组,microRNA(miR),结肠,直肠,筛选,肿瘤,和早期检测。包括使用首先处理血红蛋白的定量FIT的基于家庭的收集样品的研究。一位作者审查了所有文章;第二位作者完成了20%的全文审核,以确保遵守资格标准。
结果:广泛搜索产生了1669项研究,应用资格标准确定了18项相关研究。多种蛋白质,DNA/RNA,和微生物组生物标志物(特别是触珠蛋白,miR-16,miR-27a-3p,miR-92a,miR-148a-3p,miR-223,miR-421,let-7b-5p,和Tyzzerella4)与结直肠肿瘤有关。此外,研究强调了用于临床使用的生物标志物的短期稳定性和用于研究目的的长期稳定性.
结论:本范围综述总结了FIT残留缓冲液中生物标志物的稳定性及其与结直肠肿瘤的相关性的研究框架和进展,以指导进一步的确证研究以加强结直肠癌筛查的机会。
The faecal immunochemical test (FIT) is an inexpensive and convenient modality to screen for colorectal cancer. However, its one-time sensitivity for detecting colorectal cancer and cancer precursors is limited. There is growing interest in using the non-haemoglobin contents of FIT residual buffer to enhance colonic neoplasia detection.
To establish from the literature a framework to catalogue candidate biomarkers within FIT residual buffer for non-invasive colorectal cancer screening.
The search strategy evaluated PubMed, Scopus, Web of Science, Embase, and Google Scholar for publications through 25 October 2023, with search terms including FIT, buffer, OC-sensor, biomarkers, microbiome, microRNA (miR), colon, rectum, screening, neoplasm, and early detection. Studies employing home-based collection samples using quantitative FIT first processed for haemoglobin were included. One author reviewed all articles; a second author completed a 20% full-text audit to ensure adherence to eligibility criteria.
A broad search yielded 1669 studies and application of eligibility criteria identified 18 relevant studies. Multiple protein, DNA/RNA, and microbiome biomarkers (notably haptoglobin, miR-16, miR-27a-3p, miR-92a, miR-148a-3p, miR-223, miR-421, let-7b-5p, and Tyzzerella 4) were associated with colorectal neoplasia. Furthermore, studies highlighted the short-term stability of biomarkers for clinical use and long-term stability for research purposes.
This scoping review summarises the framework and progress of research on stability of biomarkers in FIT residual buffer and their associations with colorectal neoplasia to guide opportunities for further confirmatory studies to enhance colorectal cancer screening.