METHODS: Eight patients were included in this prospective pilot study: three patients with LNs and five with NMCs. Single voxel PRESS MRS of the tumors were acquired with 3 T MRI. MRS data were processed with LCModel version 6.3-1J using the internal \"lipid-8\" basis set. From individual lipid peak and water content measurements, total fatty acid molecules (TFAM), unsaturated fatty acid molecules (UFAM), and glycerol molecules (GM) were computed and analyzed, as well as ratios of UFAM/TFAM, TFAM/GM, and a fatty-acid chain-length index (CLI).
RESULTS: The LN group included two men and one woman (average age 58.3 years); the NMC group included two men and three women (average age 20.4 years). Lipid composition analysis showed that LN had considerably more fat than NMC: TFAM: LN = 15.29 vs NMC = 7.14; UFAM: LN = 4.48 vs NMC = 2.63; GM: LN = 5.20 vs NMC = 1.02. Both tumors had a similar fraction of unsaturated fatty acids: UFAM/TFAM: LN = 0.29 vs NMC = 0.37. LN had the usual number of FA molecules/glycerol molecule, while NMC had considerably more: TFAM/GM: LN = 2.94 vs NMC = 6.98. Finally, average FA chains were longer in NMC: CLI: LN = 17.39 vs NMC = 22.55.
CONCLUSIONS: Our analysis suggests measurable differences in the amount and composition of lipid in LN and NMC. While a larger, statistically powered study is needed, these initial findings may be helpful to properly diagnose ambiguous cases and thereby avoid surgical intervention such as biopsy.
方法:这项前瞻性试点研究包括8名患者:3名LNS患者和5名NMC患者。用3TMRI获取肿瘤的单体素PRESSMRS。使用内部“脂质-8”基础集用LCPModel版本6.3-1J处理MRS数据。从单个脂质峰值和含水量测量来看,总脂肪酸分子(TFAM),不饱和脂肪酸分子(UFAM),和甘油分子(GM)进行了计算和分析,以及UFAM/TFAM的比率,TFAM/GM,和脂肪酸链长指数(CLI)。
结果:LN组包括两名男性和一名女性(平均年龄58.3岁);NMC组包括两名男性和三名女性(平均年龄20.4岁)。脂质组成分析表明,LN的脂肪明显多于NMC:TFAM:LN=15.29vsNMC=7.14;UFAM:LN=4.48vsNMC=2.63;GM:LN=5.20vsNMC=1.02。两种肿瘤具有相似的不饱和脂肪酸分数:UFAM/TFAM:LN=0.29对NMC=0.37。LN具有通常数量的FA分子/甘油分子,而NMC有更多:TFAM/GM:LN=2.94vsNMC=6.98。最后,NMC:CLI:LN=17.39vsNMC=22.55的平均FA链更长。
结论:我们的分析表明LN和NMC中脂质的量和组成存在可测量的差异。虽然更大,需要统计学支持的研究,这些初步发现可能有助于正确诊断模棱两可的病例,从而避免手术干预,如活组织检查.