PDF(Pubmed)
Abstract：
OBJECTIVE: The purpose of this pilot porcine cadaver study was to evaluate the feasible temperature thresholds, which affect osteocyte viability and bone matrix in a preclinical setup, assessing the potential of thermal necrosis for implant removal for further in vivo investigations.
METHODS: After implant bed preparation in the upper and lower jaw, temperature effects on the bone were determined, using two tempering pistons with integrated thermocouples. To evaluate threshold temperature and time intervals leading to bone necrosis, one piston generated warm temperatures at 49 to 56 °C for 10 s and the other generated cold temperatures at 5 to 1 °C for 30 s. Effects were assessed by a semi-quantitative, histomorphometrical scoring system, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM).
RESULTS: The bone matrix was significantly degenerated starting at 51 °C for 10 s and 5 °C for 30 s. The osteocyte condition indicated significant bone damage beginning at cold temperatures of 2 °C. Temperature inputs starting at 53 °C led to decalcification and swollen mitochondria, which lost the structure of their inner cristae.
CONCLUSIONS: This study identified temperatures and durations, in both heat and cold, so that the number of samples may be kept low in further studies regarding temperature-induced bone necrosis. Levels of 51 °C for 10 s and 5 °C for 30 s have presented significant matrix degeneration.
CONCLUSIONS: Temperature thresholds, potentially leading to thermo-explantation of dental implants and other osseointegrated devices, were identified.
METHODS: After implant bed preparation in the upper and lower jaw, temperature effects on the bone were determined, using two tempering pistons with integrated thermocouples. To evaluate threshold temperature and time intervals leading to bone necrosis, one piston generated warm temperatures at 49 to 56 °C for 10 s and the other generated cold temperatures at 5 to 1 °C for 30 s. Effects were assessed by a semi-quantitative, histomorphometrical scoring system, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM).
RESULTS: The bone matrix was significantly degenerated starting at 51 °C for 10 s and 5 °C for 30 s. The osteocyte condition indicated significant bone damage beginning at cold temperatures of 2 °C. Temperature inputs starting at 53 °C led to decalcification and swollen mitochondria, which lost the structure of their inner cristae.
CONCLUSIONS: This study identified temperatures and durations, in both heat and cold, so that the number of samples may be kept low in further studies regarding temperature-induced bone necrosis. Levels of 51 °C for 10 s and 5 °C for 30 s have presented significant matrix degeneration.
CONCLUSIONS: Temperature thresholds, potentially leading to thermo-explantation of dental implants and other osseointegrated devices, were identified.
摘要：
目的：本试点猪尸体研究的目的是评估可行的温度阈值，在临床前设置中影响骨细胞活力和骨基质，评估热坏死的潜在植入物移除进一步的体内研究。
方法：在上下颌植入床准备后，确定了温度对骨骼的影响，使用两回火活塞集成热电偶。为了评估导致骨坏死的阈值温度和时间间隔，一个活塞在49至56°C下产生10s的温暖温度，另一个在5至1°C下产生30s的寒冷温度。组织形态统计评分系统，扫描电子显微镜（SEM），能量色散X射线光谱（EDX），和透射电子显微镜（TEM）。
结果：骨基质在51°C下10s和5°C下30s开始显着变性。骨细胞状况表明在2°C的低温下开始出现明显的骨损伤。从53°C开始的温度输入导致线粒体脱钙和肿胀，失去了它们内部的结构。
结论：这项研究确定了温度和持续时间，在高温和寒冷中，因此，在关于温度诱导的骨坏死的进一步研究中，样品的数量可以保持较低。51°C持续10s和5°C持续30s的水平表现出明显的基质变性。
结论：温度阈值，可能导致牙科植入物和其他骨整合装置的热移植，已确定。
方法：在上下颌植入床准备后，确定了温度对骨骼的影响，使用两回火活塞集成热电偶。为了评估导致骨坏死的阈值温度和时间间隔，一个活塞在49至56°C下产生10s的温暖温度，另一个在5至1°C下产生30s的寒冷温度。组织形态统计评分系统，扫描电子显微镜（SEM），能量色散X射线光谱（EDX），和透射电子显微镜（TEM）。
结果：骨基质在51°C下10s和5°C下30s开始显着变性。骨细胞状况表明在2°C的低温下开始出现明显的骨损伤。从53°C开始的温度输入导致线粒体脱钙和肿胀，失去了它们内部的结构。
结论：这项研究确定了温度和持续时间，在高温和寒冷中，因此，在关于温度诱导的骨坏死的进一步研究中，样品的数量可以保持较低。51°C持续10s和5°C持续30s的水平表现出明显的基质变性。
结论：温度阈值，可能导致牙科植入物和其他骨整合装置的热移植，已确定。
