Abstract：
Antibodies are useful for localizing glycoprotein antigens in histochemistry, but they do not differentiate glycoforms in tissue sections because conventional antibodies recognize only protein epitopes rather than glycans. Glycan epitopes are recognized by lectins, which are found, occasionally, to inhibit antigen-antibody reaction in a glycoform-specific manner (lectin inhibition). Here we describe the application of lectin inhibition to immunohistochemistry for visualizing a glycoform in a tissue section.
摘要：
抗体可用于在组织化学中定位糖蛋白抗原，但它们不能区分组织切片中的糖型，因为常规抗体只识别蛋白质表位而不是聚糖。聚糖表位被凝集素识别，被发现，偶尔,以糖型特异性方式抑制抗原-抗体反应(凝集素抑制)。在这里，我们描述了凝集素抑制在免疫组织化学中的应用，用于可视化组织切片中的糖型。
