对Jacalin的聚糖结合特异性进行了研究,RCA120,SBA,PHA-L,PHA-E,WGA,UEA,AAL,LTL,LEL,SNA,DSA,LCA,MAH和ConA,在组织化学中广泛使用的凝集素。应用基于寡糖和多糖的聚糖阵列。仅对15种凝集素中的6种证实了预期的特异性,并且一些凝集素的聚糖结合谱比普遍接受的显著更宽。WGA,LEL和DSA被称为壳寡糖特异性,出乎意料的是多反应性的,以与壳二糖相同的亲和力与其他聚糖结合,ABH抗原和寡糖胺(未取代和唾液酸化)。SBA,除了预期与末端GalNAcα的聚糖结合外,对GM1神经节苷脂也有很高的亲和力。与Neu5Acα2-3Galβ1-3GalNAca相比,MAH对多种硫酸化聚糖表现出更高的亲和力。与普遍观点相反,LCA显示与(GlcNAcβ1-2Manα1)2-3,6-Manβ1-4GlcNAcβ1-4GlcNAcN-聚糖的最大结合,而与相应的Gal或Neu5Ac终止版本没有相互作用。观察到的某些凝集素的多反应性令人怀疑它们在通过组织化学研究准确确定特定聚糖结构的存在中的用途。然而,在凝集素阵列的帮助下,比较健康和患病个体的血清可以很容易地建立糖基化模式和推定聚糖身份的差异,这以后可以用更准确的结构分析方法来澄清。
Glycan-binding specificity was studied for Jacalin, RCA 120, SBA, PHA-L, PHA-E, WGA, UEA, AAL, LTL, LEL, SNA, DSA, LCA, MAH and Con A, lectins widely used in histochemistry. Oligosaccharide- and polysaccharide-based glycan arrays were applied. Expected specificity was confirmed for only 6 of the 15 lectins and the glycan binding profiles of some lectins were dramatically broader than generally accepted. WGA, LEL and DSA known as chitooligosaccharide-specific, were unexpectedly polyreactive, binding to other glycans with the same affinity as to chitobiose, ABH antigens and oligolactosamines (unsubstituted and sialylated). SBA, in addition to expected binding to glycans with terminal GalNAcα, also had high affinity for the GM1 ganglioside. MAH demonstrated much higher affinity to a variety of sulfated glycans compared to Neu5Acα2-3Galβ1-3GalNAcα. Contrary to the common view, LCA demonstrated the maximum binding to (GlcNAcβ1-2Manα1)2-3,6-Manβ1-4GlcNAcβ1-4GlcNAc N-glycan, while it had no interaction with corresponding Gal or Neu5Ac terminated versions. This observed polyreactivity of some lectins casts doubt on their use in accurately determining the presence of a specific glycan structure by histochemical studies. However, comparisons of sera from healthy and diseased individuals with help of a lectin array can easily establish differences in glycosylation patterns and presumptive glycan identities, which can later be clarified using more accurate methods of structural analysis.