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Abstract：
C-type lectins (CTLs) have received widespread attention in animal immune responses. In the present study, two CTLs (ToCTL1 and ToCTL2) were identified from obscure puffer Takifugu obscurus. The open reading frames of ToCTL1 and ToCTL2 were 687 and 1,380 bp, respectively. The predicted ToCTL1 and ToCTL2 proteins contained a single transmembrane region and one typical carbohydrate recognition domain (CRD). Quantitative real-time polymerase chain reaction detected ToCTL1 and ToCTL2 transcripts in all examined tissues, with high levels in the intestine and kidney, and their expression levels were remarkably altered upon Vibrio harveyi and Aeromonas hydrophila infection. The recombinant proteins ToCTL1-CRD and ToCTL2-CRD agglutinated the Gram-negative and Gram-positive bacteria in a Ca2+-dependent manner. rToCTL1-CRD and rToCTL2-CRD exhibited evident binding activities against seven kinds of bacteria and polysaccharides (lipopolysaccharide and peptidoglycan) in a Ca2+-independent manner. Moreover, rToCTL1-CRD and rToCTL2-CRD could inhibit the growth of four types of bacteria in vitro. These findings collectively demonstrated that ToCTL1 and ToCTL2 could be involved in host defense against bacterial infection in T. obscurus.
摘要：
C型凝集素(CTL)在动物免疫应答中受到广泛关注。在本研究中,从晦涩的河豚Takifugu暗箱中鉴定出两个CTL（ToCTL1和ToCTL2）。ToCTL1和ToCTL2的开放阅读框为687和1380bp,分别。预测的ToCTL1和ToCTL2蛋白包含单个跨膜区和一个典型的碳水化合物识别结构域(CRD)。定量实时聚合酶链反应检测到所有检查组织中的ToCTL1和ToCTL2转录本，肠道和肾脏中的高水平，在哈氏弧菌和嗜水气单胞菌感染后，它们的表达水平显着改变。重组蛋白ToCTL1-CRD和ToCTL2-CRD以Ca2依赖性方式凝集革兰氏阴性和革兰氏阳性细菌。rToCTL1-CRD和rToCTL2-CRD以不依赖Ca2的方式对7种细菌和多糖（脂多糖和肽聚糖）表现出明显的结合活性。此外，rToCTL1-CRD和rToCTL2-CRD可以抑制4种细菌的体外生长。这些发现共同证明了ToCTL1和ToCTL2可能参与宿主防御暗黑T.
