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Abstract：
A sulfated galactan LP-G2 was isolated and purified from red alga, Pyropia haitanensis. It was a branched polysaccharide with average molecular weight about 8381 Da, composed of Gal, Glc, GalA and Ara. The structure of LP-G2 was determined using IR and NMR spectroscopy. It was composed of →4)-β-d-galactose→4)-α-l-galactose-6- sulfate segments, with β-d-Glc and α-d-galactose unit substituted at the 6-position of α-l-galactose. Functional analysis showed that LP-G2 inhibited complement activation on both the classic and alternative pathways with CH50 value of 3.08 ± 0.25 mg/mL and AP50 value of 2.23 ± 0.20 mg/mL, respectively. Preliminary mechanism studies by using complement component depleted-sera indicated that LP-G2 selectively interacts with C1q, C2, C4 and C9. The results suggested that LP-G2 could be of potential benefits in treatment of the complement associated diseases.
摘要：
从红藻中分离纯化硫酸化半乳聚糖LP-G2,海地足。它是一种支链多糖，平均分子量约为8381Da，由Gal组成,Glc,Gala和Ara.使用IR和NMR光谱法确定LP-G2的结构。它由→4）-β-d-半乳糖→4）-α-1-半乳糖-6-硫酸酯段组成，在α-1-半乳糖的6位取代有β-d-Glc和α-d-半乳糖单元。功能分析显示，LP-G2抑制经典和替代途径的补体激活，CH50值为3.08±0.25mg/mL，AP50值为2.23±0.20mg/mL。分别。通过使用补体成分耗尽的血清的初步机制研究表明，LP-G2选择性地与C1q相互作用，C2、C4和C9。结果表明，LP-G2可能在治疗补体相关疾病方面具有潜在的益处。
