伤口在医疗保健方面构成了巨大的挑战,需要探索创新的组织愈合解决方案。传统的伤口敷料表现出缺点,造成组织损伤和阻碍自然愈合。使用微波(MW)-)辅助技术,我们设想了一种新型的水凝胶(Hg)支架来应对这些挑战。这种水凝胶支架是通过合成一种pH响应性交联材料,特别是刺槐豆胶接枝的聚(丙烯酰胺-共-丙烯酸)[LBG-g-聚(AAm-co-AAc)],以实现c-藻蓝蛋白(C-Pc)的持续释放。通过调整各种合成参数对合成的LBG-g-poly(AAm-co-AAc)进行微调,包括单体的浓度,反应时间,和MW辐照强度,最大限度地提高交联LBG接枝产物的收率,提高C-Pc的包封率。在其合成之后,LBG-g-poly(AAm-co-AAc)使用先进技术进行了彻底表征,像XRD,TGA,FTIR,NMR,和SEM,分析其结构和化学性质。此外,该研究检查了基于LBG-g-聚(AAm-co-AAc)的水凝胶(HgCPcLBG)的体外C-Pc释放曲线。研究结果表明,C-Pc的最大释放(64.12±2.69%)在pH7.4超过48小时。HgCPcLBG表现出增强的抗氧化性能和与血液的相容性。体内研究证实伤口加速闭合,和ELISA结果显示炎症标志物减少(IL-6,IL-1β,TNF-α)在治疗的皮肤组织内,提示对损伤修复的积极影响。已开发出一种低成本且环保的方法来创建LBG-g-聚(AAm-co-AAc)和HgCPcLBG。该方法实现了C-Pc的持续释放,这可能是伤口护理技术向前迈出的重要一步。
Wounds pose a formidable challenge in healthcare, necessitating the exploration of innovative tissue-healing solutions. Traditional wound dressings exhibit drawbacks, causing tissue damage and impeding natural healing. Using a Microwave (MW)-)-assisted technique, we envisaged a novel hydrogel (Hg) scaffold to address these challenges. This hydrogel scaffold was created by synthesizing a pH-responsive crosslinked material, specifically locust bean gum-grafted-poly(acrylamide-co-acrylic acid) [LBG-g-poly(AAm-co-AAc)], to enable sustained release of c-phycocyanin (C-Pc). Synthesized LBG-g-poly(AAm-co-AAc) was fine-tuned by adjusting various synthetic parameters, including the concentration of monomers, duration of reaction, and MW irradiation intensity, to maximize the yield of crosslinked LBG grafted product and enhance encapsulation efficiency of C-Pc. Following its synthesis, LBG-g-poly(AAm-co-AAc) was thoroughly characterized using advanced techniques, like XRD, TGA, FTIR, NMR, and SEM, to analyze its structural and chemical properties. Moreover, the study examined the in-vitro C-Pc release profile from LBG-g-poly(AAm-co-AAc) based hydrogel (HgCPcLBG). Findings revealed that the maximum release of C-Pc (64.12 ± 2.69 %) was achieved at pH 7.4 over 48 h. Additionally, HgCPcLBG exhibited enhanced antioxidant performance and compatibility with blood. In vivo studies confirmed accelerated wound closure, and ELISA findings revealed reduced inflammatory markers (IL-6, IL-1β, TNF-α) within treated skin tissue, suggesting a positive impact on injury repair. A low-cost and eco-friendly approach for creating LBG-g-poly(AAm-co-AAc) and HgCPcLBG has been developed. This method achieved sustained release of C-Pc, which could be a significant step forward in wound care technology.