%0 Journal Article
%T Anti-complementary activity of a degraded sulfated heterogalactan from red alga Pyropia haitanensis.
%A Zhang Z
%A Wang X
%A Lv F
%A Xie X
%A Zhang S
%A Cai C
%A Jia R
%A Pan Y
%A Liu F
%J Int J Biol Macromol
%V 147
%N 0
%D Mar 2020 15
%M 31926924
%F 8.025
%R 10.1016/j.ijbiomac.2020.01.045
%X A sulfated galactan LP-G2 was isolated and purified from red alga, Pyropia haitanensis. It was a branched polysaccharide with average molecular weight about 8381 Da, composed of Gal, Glc, GalA and Ara. The structure of LP-G2 was determined using IR and NMR spectroscopy. It was composed of →4)-β-d-galactose→4)-α-l-galactose-6- sulfate segments, with β-d-Glc and α-d-galactose unit substituted at the 6-position of α-l-galactose. Functional analysis showed that LP-G2 inhibited complement activation on both the classic and alternative pathways with CH50 value of 3.08 ± 0.25 mg/mL and AP50 value of 2.23 ± 0.20 mg/mL, respectively. Preliminary mechanism studies by using complement component depleted-sera indicated that LP-G2 selectively interacts with C1q, C2, C4 and C9. The results suggested that LP-G2 could be of potential benefits in treatment of the complement associated diseases.