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Abstract：
Ilex species have been consumed traditionally as medicinal teas worldwide. Though the presence of caffeine has been reported in several species, little is known about secondary-metabolite diversity within and among these taxa. Leaf samples of Ilex guayusa, Ilex paraguariensis, and Ilex vomitoria were analyzed by liquid chromatography-mass spectrometry and comparative metabolite profiling with Ilex cassine and other Ilex species to identify chemotaxonomic markers, delimit species, and provide an assessment of chemodiversity. Purine alkaloids were detected and quantified in I. guayusa, I. paraguariensis, and I. vomitoria. Reports of caffeine for I. cassine were not corroborated, suggesting that I. vomitoria was the traditional source of the Native North American tea yaupon. The tetramethyluric acid, theacrine, was detected for the first time in the genus Ilex as a low-level chemotaxonomic marker in I. vomitoria samples. Chemotaxonomy and metabolomics support a close relationship for caffeine-containing Ilex species.
摘要：
Ilex物种在全世界传统上被用作药用茶。虽然咖啡因的存在已经在几个物种中被报道，对这些分类单元内部和之间的次生代谢物多样性知之甚少。Ilexguayusa的叶子样本，Paraguariensis,通过液相色谱-质谱分析和与Ilex卡辛和其他Ilex物种的比较代谢物谱分析，以鉴定化学分类标记，划定物种，并提供化学多样性评估。嘌呤生物碱在I.guayusa中检测和定量，一、巴拉圭，还有我的呕吐.卡辛碱的咖啡因报告没有得到证实，这表明I.vomitoria是北美原住民茶的传统来源。四甲基尿酸，theacrine,在I.vomitoria样品中首次在Ilex属中检测到作为低水平化学分类学标记。化学分类学和代谢组学支持含咖啡因的Ilex物种的密切关系。
