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Abstract：
There have been various studies about the acellular nerve allograft (ANA) as the alternative of autologous nerve graft in the treatment of peripheral nerve defects. As well as the decellularization process methods of ANA, the various enhancement methods of regeneration of the grafted ANA were investigated. The chondroitin sulfate proteoglycans (CSPGs) inhibit the action of laminin which is important for nerve regeneration in the extracellular matrix of nerve. Chondroitinase ABC (ChABC) has been reported that it enhances the nerve regeneration by degradation of CSPGs. The present study compared the regeneration of ANA between the processed without ChABC group and the processed with ChABC group in a rat sciatic nerve 15 mm gap model. At 12 weeks postoperatively, there was not a significant difference in the histomorphometric analysis. In the functional analysis, there were no significant differences in maximum isometric tetanic force, wet muscle weight of tibialis anterior. The processed without ChABC group had better result in ankle contracture angle significantly. In conclusion, there were no significant differences in the regeneration of ANA between the processed without ChABC group and the processed with ChABC group.
摘要：
关于去细胞同种异体神经移植（ANA）作为自体神经移植治疗周围神经缺损的替代方法，已经有各种研究。以及ANA的去细胞化过程方法，研究了各种增强接枝ANA再生的方法。硫酸软骨素蛋白聚糖（CSPGs）抑制层粘连蛋白的作用，层粘连蛋白对神经细胞外基质中的神经再生很重要。据报道，软骨素酶ABC（ChABC）通过降解CSPGs来增强神经再生。本研究在大鼠坐骨神经15mm间隙模型中比较了无ChABC处理组和有ChABC处理组之间ANA的再生。术后12周，组织形态计量学分析无显著差异.在功能分析中，最大等距强直力没有显着差异，胫骨前肌湿重。未处理ChABC组的踝关节挛缩角明显较好。总之,未处理ChABC组与处理ChABC组之间的ANA再生没有显着差异。
