目的:本研究旨在评估全身/局部使用褪黑素和醋酸格拉替雷对创伤性神经损伤模型再生的影响。
方法:将42只雄性Wistar白化病大鼠随机分为6组:健康对照组(第1组),受伤的对照组(第2组),局部褪黑素(第3组),全身性褪黑激素(第4组),局部醋酸格拉替雷(第5组),和全身性醋酸格拉替雷(第6组)。在所有组中,手术后和处死前获得面神经的肌电图记录,处死后对受损的神经区域进行组织病理学检查。
结果:在电生理评估中,与治疗组相比,对照组在手术后波幅和潜伏期延长方面的下降幅度最大.此外,退行性轴突数量显著减少,水肿区,与损伤对照组相比,在所有治疗组中观察到纤维化区域以及轴突表面积的显着增加。
结论:虽然醋酸格拉替雷和褪黑素对外伤性面神经损伤的再生都有益,可以得出结论,与醋酸格拉替雷和局部使用两种药物相比,全身使用褪黑激素可以产生更积极的结果。
OBJECTIVE: This study aimed to evaluate the effect of systemic/local use of melatonin and glatiramer acetate on regeneration in traumatic nerve injury models.
METHODS: A total of 42 male Wistar albino rats were randomly divided into 6 groups: healthy control (Group 1), injured control (Group 2), local melatonin (Group 3), systemic melatonin (Group 4), local glatiramer acetate (Group 5), and systemic glatiramer acetate (Group 6). In all groups, electromyography recordings of the facial nerve were obtained after surgery and before sacrifice, and the damaged nerve region was histopathologically examined after sacrifice.
RESULTS: In the electrophysiological evaluation, the control group had the greatest decrease in amplitude and extension in latency time following surgery than the treatment groups. Furthermore, a significant decrease in the degenerative axon count, edematous areas, and fibrotic areas as well as a significant increase in axonal surface areas was observed in all the treatment groups compared with the damage control group.
CONCLUSIONS: Although both glatiramer acetate and melatonin are beneficial in regeneration in traumatic facial nerve injuries, it can be concluded that systemic use of melatonin can yield more positive results than glatiramer acetate and local use of both two drugs.