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Abstract：
BACKGROUND: To improve osseointegration and enhance the success rate of implanted biomaterials, the surface modification technology of bone implants has developed rapidly. Intensive research on osteoimmunomodulation has shown that the surfaces of implants should possess favorable osteoimmunomodulation to facilitate osteogenesis.
METHODS: A novel, green and efficient phase-transited lysozyme (PTL) technique was used to prime titanium discs with a positive charge. In addition, sodium hyaluronate (HA) and self-assembled type I collagen containing aspirin (ASA) nanoparticles were decorated on PTL-primed Ti discs via electrostatic interaction.
RESULTS: The behaviors of bone marrow stromal cells (BMSCs) on the Ti disc surfaces containing ASA were analyzed in different conditioned media (CM) generated by macrophages. Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification. After implantation in vivo, the ASA-modified implant can significantly contribute to bone formation around the implant, which mirrors the evaluation in vitro.
CONCLUSIONS: This study highlights the significant effects of appropriate surface characteristics on the regulation of osteogenesis and osteoimmunomodulation around an implant. Implant modification with ASA potentially provides superior strategies for the surface modification of biomaterials.
METHODS: A novel, green and efficient phase-transited lysozyme (PTL) technique was used to prime titanium discs with a positive charge. In addition, sodium hyaluronate (HA) and self-assembled type I collagen containing aspirin (ASA) nanoparticles were decorated on PTL-primed Ti discs via electrostatic interaction.
RESULTS: The behaviors of bone marrow stromal cells (BMSCs) on the Ti disc surfaces containing ASA were analyzed in different conditioned media (CM) generated by macrophages. Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification. After implantation in vivo, the ASA-modified implant can significantly contribute to bone formation around the implant, which mirrors the evaluation in vitro.
CONCLUSIONS: This study highlights the significant effects of appropriate surface characteristics on the regulation of osteogenesis and osteoimmunomodulation around an implant. Implant modification with ASA potentially provides superior strategies for the surface modification of biomaterials.
摘要：
背景：为了改善骨整合并提高植入生物材料的成功率，骨种植体的表面改性技术发展迅速。对骨免疫调节的深入研究表明，植入物的表面应具有良好的骨免疫调节作用，以促进成骨。
方法：小说，绿色高效的相变溶菌酶（PTL）技术用于准备带有正电荷的钛圆盘。此外,透明质酸钠（HA）和含有阿司匹林（ASA）的自组装I型胶原蛋白的纳米颗粒通过静电相互作用装饰在PTL引发的Ti圆盘上。
结果：在巨噬细胞产生的不同条件培养基（CM）中分析了骨髓基质细胞（BMSCs）在含有ASA的Ti椎间盘表面上的行为。此外,在体外实验中研究了巨噬细胞在不同钛盘表面的炎症相关细胞因子的分泌,这表明含有ASA的Ti表面不仅支持迁移，与未经表面修饰的Ti盘相比，BMSCs的增殖和分化也降低了巨噬细胞的炎症反应。植入体内后，ASA修饰的植入物可以显著促进植入物周围的骨形成，反映了体外评估。
结论：本研究强调了适当的表面特征对植入物周围成骨和骨免疫调节的调节的显著影响。用ASA进行的植入物改性可能为生物材料的表面改性提供了优越的策略。
方法：小说，绿色高效的相变溶菌酶（PTL）技术用于准备带有正电荷的钛圆盘。此外,透明质酸钠（HA）和含有阿司匹林（ASA）的自组装I型胶原蛋白的纳米颗粒通过静电相互作用装饰在PTL引发的Ti圆盘上。
结果：在巨噬细胞产生的不同条件培养基（CM）中分析了骨髓基质细胞（BMSCs）在含有ASA的Ti椎间盘表面上的行为。此外,在体外实验中研究了巨噬细胞在不同钛盘表面的炎症相关细胞因子的分泌,这表明含有ASA的Ti表面不仅支持迁移，与未经表面修饰的Ti盘相比，BMSCs的增殖和分化也降低了巨噬细胞的炎症反应。植入体内后，ASA修饰的植入物可以显著促进植入物周围的骨形成，反映了体外评估。
结论：本研究强调了适当的表面特征对植入物周围成骨和骨免疫调节的调节的显著影响。用ASA进行的植入物改性可能为生物材料的表面改性提供了优越的策略。
