PDF(Sci-hub)
Abstract：
Serodiagnosis of human anisakidosis is presently hampered by the current lack of standardised serological assays that allow sensitive and specific detection of Anisakidae-specific antibodies in human patients. In the present study, we comparatively evaluated the diagnostic value (by IgG-ELISA) of excretory-secretory antigens (ESAgs) of Anisakis simplex, Pseudoterranova decipiens and Contracaecum osculatum, representing the most frequently found genera responsible for human infection. In addition, we tested also a mix of the three ES preparations (Mix-ESAgs) as well as two recombinant allergens of A. simplex, rAni s 1 and rAni s 7. ES antigen from C. osculatum yielded the best diagnostic performance in IgG-ELISA-based serodiagnosis of the Spanish anisakidosis patients investigated in this study (relative serodiagnostic sensitivity 100%; specificity 89%) as compared to A. simplex ES-antigen (93% versus 57%) and P. decipiens (67% versus 93%) or a mix of the three ES antigens (100% versus 44%), respectively. Cross-reactions of C. osculatum ES antigen with serum-antibodies from patients suffering from other helminth infections were rare and were exclusively found with few sera from toxocariasis, ascariasis, and filariasis patients. The two recombinant allergens rAni s 1 and rAni s 7 did not prove sufficiently sensitive and specific in order to justify a further evaluation of these antigens regarding their suitability in IgG-ELISA-based serodiagnosis of human anisakidosis. In conclusion, the C. osculatum-ESAg-ELISA remains as key candidate to be further assessed for the serodiagnosis of symptomatic anisakidosis in different endemic regions.
摘要：
目前，由于缺乏标准化的血清学测定，无法对人类患者中的异株科特异性抗体进行灵敏和特异性的检测，因此阻碍了人类异株病的血清诊断。在本研究中,我们比较评估了诊断价值（通过IgG-ELISA）的排泄分泌抗原（ESA）的单纯异尖，假性蜕膜和角囊，代表最常见的人类感染属。此外,我们还测试了三种ES制剂（Mix-ESA）的混合物以及两种重组的A.simplex过敏原，rAnis1和rAnis7。在本研究中调查的西班牙念珠菌病患者的基于IgG-ELISA的血清诊断中，来自C.osculatum的ES抗原与单纯型ES抗原（93％对57％）和蜕膜假单胞菌（67％对93％）或三种ES抗原的混合（100％对44％）相比，具有最佳的诊断性能。分别。C.osculatumES抗原与患有其他蠕虫感染的患者的血清抗体的交叉反应很少见，并且仅在很少的弓形虫病血清中发现。蛔虫病,和丝虫病患者。两种重组变应原rAnis1和rAnis7没有证明足够的敏感性和特异性，以证明进一步评估这些抗原在基于IgG-ELISA的人类anisakidosis血清诊断中的适用性。总之,C.osculatum-ESAg-ELISA仍然是需要进一步评估的关键候选指标，用于在不同流行区域的症状性anisakidosis的血清诊断。
