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Abstract：
N-acetyl-L-cysteine (NAC) is a derivative of the sulphur-containing amino acid L-cysteine with potential anti-aging properties. We studied 3 Drosophila species with contrast longevity differences (D. virilis is longest-lived, D. kikkawai is shortest-lived and D. melanogaster has moderate lifespan) to test the effects of NAC at 8 different concentrations (from 10 nM to 100 mM) on the lifespan, stress-resistance and locomotor activity. Except the adverse effects of highest (10 mM and 100 mM) concentrations NAC demonstrated sexually opposite and male-biased effects on Drosophila lifespan, stress-resistance and locomotor activity and not satisfied the criteria of a geroprotector in terms of the reproducibility of lifespan extending effects in different model organisms. The concentration- and sex-dependent changes in the relative expression levels of the antioxidant genes (Cat/CG6871 and Sod1/CG11793) and genes involved in hydrogen sulfide biosynthesis (Cbs/CG1753, Eip55E/CG5345 and Nfs1/CG12264) suggest the involvement of hormetic mechanisms in the geroprotective effects of NAC.
摘要：
N-乙酰基-L-半胱氨酸（NAC）是含硫氨基酸L-半胱氨酸的衍生物，具有潜在的抗衰老特性。我们研究了3种果蝇，具有对比寿命差异（D。维里利斯寿命最长,D.kikkawai寿命最短，D.melanogaster具有中等寿命），以测试8种不同浓度（从10nM到100mM）的NAC对寿命的影响，抗压力和运动活动。除了最高浓度（10mM和100mM）的NAC对果蝇寿命表现出相反性和男性偏见的影响外，抗逆性和运动活性，并且在不同模型生物中寿命延长效应的可重复性方面不满足年龄保护者的标准。抗氧化剂基因（Cat/CG6871和Sod1/CG11793）和参与硫化氢生物合成的基因（Cbs/CG1753，Eip55E/CG5345和Nfs1/CG12264）的相对表达水平的浓度和性别依赖性变化表明参与了NAC的衰老保护作用。
