RNA-Seq analysis was used for gene expression profiling of persister cells in nutrient-rich medium (persister TN) compared with untreated cells (non-persister). The results were confirmed using reverse transcription quantitative PCR (RT-qPCR).
Functional genes associated with the persister population were identified in multiple systems, such as heat-shock-related stress response, cell wall synthesis, ATP-binding cassette (ABC) transport system, phosphotransferase system (PTS) and SOS/DNA repair.
This study pointed to genetic regulation of persister cells exposed to lethal nisin concentrations and provides some insight into possible mechanisms of impeding bacterial persistence.
RNA-Seq分析用于在营养丰富的培养基(persisterTN)中与未处理的细胞(非persister)相比的persister细胞的基因表达谱。使用逆转录定量PCR(RT-qPCR)确认结果。
在多个系统中鉴定出与持久群体相关的功能基因,如热休克相关的应激反应,细胞壁合成,ATP结合盒(ABC)转运系统,磷酸转移酶系统(PTS)和SOS/DNA修复。
这项研究指出了暴露于致死性乳酸链球菌素浓度的持久性细胞的遗传调控,并提供了一些阻碍细菌持久性的可能机制的见解。