%0 Journal Article
%T Transcriptomic study on persistence and survival of Listeria monocytogenes following lethal treatment with nisin.
%A Wu S
%A Yu PL
%A Wheeler D
%A Flint S
%J J Glob Antimicrob Resist
%V 15
%N 0
%D 12 2018
%M 29933119
%F 4.349
%R 10.1016/j.jgar.2018.06.003
%X The aim of this study was to determine gene expression associated with the persistence of a Listeria monocytogenes stationary-phase population when facing lethal nisin treatment.<BR>RNA-Seq analysis was used for gene expression profiling of persister cells in nutrient-rich medium (persister TN) compared with untreated cells (non-persister). The results were confirmed using reverse transcription quantitative PCR (RT-qPCR).<BR>Functional genes associated with the persister population were identified in multiple systems, such as heat-shock-related stress response, cell wall synthesis, ATP-binding cassette (ABC) transport system, phosphotransferase system (PTS) and SOS/DNA repair.<BR>This study pointed to genetic regulation of persister cells exposed to lethal nisin concentrations and provides some insight into possible mechanisms of impeding bacterial persistence.