{Reference Type}: Journal Article
{Title}: Transcriptomic study on persistence and survival of Listeria monocytogenes following lethal treatment with nisin.
{Author}: Wu S;Yu PL;Wheeler D;Flint S;
{Journal}: J Glob Antimicrob Resist
{Volume}: 15
{Issue}: 0
{Year}: 12 2018
{Factor}: 4.349
{DOI}: 10.1016/j.jgar.2018.06.003
{Abstract}: The aim of this study was to determine gene expression associated with the persistence of a Listeria monocytogenes stationary-phase population when facing lethal nisin treatment.<BR>RNA-Seq analysis was used for gene expression profiling of persister cells in nutrient-rich medium (persister TN) compared with untreated cells (non-persister). The results were confirmed using reverse transcription quantitative PCR (RT-qPCR).<BR>Functional genes associated with the persister population were identified in multiple systems, such as heat-shock-related stress response, cell wall synthesis, ATP-binding cassette (ABC) transport system, phosphotransferase system (PTS) and SOS/DNA repair.<BR>This study pointed to genetic regulation of persister cells exposed to lethal nisin concentrations and provides some insight into possible mechanisms of impeding bacterial persistence.