补充系统,由补体成分和补体控制蛋白组成,在先天免疫中起着至关重要的作用。补体系统分子在母体-概念界面表达,补体系统的不适当激活与人类和啮齿动物的各种不良妊娠结局有关。然而,表达式,regulation,猪母体-概念界面补体系统的功能尚未研究。在这项研究中,我们调查了表达式,本地化,和猪母体-概念界面补体系统分子的调节。补体组分和补体控制蛋白在子宫内膜中表达,早期概念,怀孕期间的绒毛尿囊组织。作用于补体激活早期的补体成分在妊娠第15天子宫内膜中的表达增加,与发情周期相比,当天的水平更高。几种补体成分和补体控制蛋白的定位在子宫内膜中是细胞类型特异性的。C1QC的表达,C2,C3,C4A,CFI,IFNG在子宫内膜外植体组织中增加ITGB2,MASP1和SERPING1。此外,在发情周期的第15天和妊娠的第15天,在子宫内膜组织和子宫潮红中检测到裂解的C3片段,怀孕第15天的水平更高。这些结果表明,在母体-概念界面上表达的猪补体系统分子通过调节先天免疫和调节妊娠期母体免疫环境在建立和维持妊娠中起重要作用。
The complement system, composed of complement components and complement control proteins, plays an essential role in innate immunity. Complement system molecules are expressed at the maternal-conceptus interface, and inappropriate activation of the complement system is associated with various adverse pregnancy outcomes in humans and rodents. However, the expression, regulation, and function of the complement system at the maternal-conceptus interface in pigs have not been studied. In this study, we investigated the expression, localization, and regulation of complement system molecules at the maternal-conceptus interface in pigs. Complement components and complement control proteins were expressed in the endometrium, early-stage conceptus, and chorioallantoic tissues during pregnancy. The expression of complement components acting on the early stage of complement activation increased in the endometrium on Day 15 of pregnancy, with greater levels on that day compared with the estrous cycle. Localization of several complement components and complement control proteins was cell-type specific in the endometrium. The expression of C1QC, C2, C3, C4A, CFI, ITGB2, MASP1, and SERPING1 was increased by IFNG in endometrial explant tissues. Furthermore, cleaved C3 fragments were detected in endometrial tissues and uterine flushings on Day 15 of the estrous cycle and Day 15 of pregnancy, with greater levels on Day 15 of pregnancy. These results suggest that complement system molecules in pigs expressed at the maternal-conceptus interface play important roles in the establishment and maintenance of pregnancy by regulating innate immunity and modulating the maternal immune environment during pregnancy.