关键词: Brain slices Ex vivo Hippocampus Neuropharmacology Organotypic culture Rat Toxicity β-Amyloid

Mesh : Amyloid beta-Peptides / toxicity Animals Animals, Newborn Hippocampus / cytology drug effects Organ Culture Techniques / methods Rats Rats, Sprague-Dawley Rats, Wistar

来  源:   DOI:10.1007/978-1-4939-7571-6_24

Abstract:
Hippocampal organotypic cultures constitute a very easy but delicate method widely used to study amyloid β-peptide toxicity. This ex vivo technique is performed on tissues isolated from newborn rats. Here, we describe a protocol for the preparation and culture of hippocampal organotypic slices that can be maintained for 14-21 days and their application to the study of amyloid β-peptide toxicity.
摘要:
暂无翻译
公众号