{Reference Type}: Journal Article {Title}: Preparation of Rat Hippocampal Organotypic Cultures and Application to Study Amyloid β-Peptide Toxicity. {Author}: Bronzuoli MR;Facchinetti R;Scuderi C; {Journal}: Methods Mol Biol {Volume}: 1727 {Issue}: 0 {Year}: 2018 暂无{DOI}: 10.1007/978-1-4939-7571-6_24 {Abstract}: Hippocampal organotypic cultures constitute a very easy but delicate method widely used to study amyloid β-peptide toxicity. This ex vivo technique is performed on tissues isolated from newborn rats. Here, we describe a protocol for the preparation and culture of hippocampal organotypic slices that can be maintained for 14-21 days and their application to the study of amyloid β-peptide toxicity.