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Abstract：
Freshwater habitat alteration and marine fisheries can affect anadromous fish species, and populations fluctuating in size elicit conservation concern and coordinated management. We describe the development and characterization of two sets of 96 single nucleotide polymorphism (SNP) assays for two species of anadromous alosine fishes, alewife and blueback herring (collectively known as river herring), that are native to the Atlantic coast of North America. We used data from high-throughput DNA sequencing to discover SNPs and then developed molecular genetic assays for genotyping sets of 96 individual loci in each species. The two sets of assays were validated with multiple populations that encompass both the geographic range and the known regional genetic stocks of both species. The SNP panels developed herein accurately resolved the genetic stock structure for alewife and blueback herring that was previously identified using microsatellites and assigned individuals to regional stock of origin with high accuracy. These genetic markers, which generate data that are easily shared and combined, will greatly facilitate ongoing conservation and management of river herring including genetic assignment of marine caught individuals to stock of origin.
摘要：
淡水栖息地的改变和海洋渔业会影响现有鱼类，人口规模的波动引发了保护问题和协调管理。我们描述了两组96个单核苷酸多态性（SNP）测定法的开发和表征，这些测定法用于两种无性系的腺苷鱼类，阿列夫和蓝背鲱鱼(统称为河鲱鱼)，原产于北美大西洋沿岸。我们使用来自高通量DNA测序的数据来发现SNP，然后开发了用于对每个物种中96个个体基因座进行基因分型的分子遗传测定。这两组试验用多个种群进行了验证，这些种群涵盖了两个物种的地理范围和已知的区域遗传种群。本文开发的SNP小组准确地解析了先前使用微卫星鉴定的蜜环鱼和蓝背鲱鱼的遗传种群结构，并以高精度将个体分配到区域起源种群。这些遗传标记，它们生成易于共享和组合的数据，将极大地促进河流鲱鱼的持续保护和管理，包括将海洋捕获的个体遗传分配给原产地。
