关键词: Area under curve Cytokinesis defect Cytotoxicity Micronucleus

Mesh : Case-Control Studies Cell Death / genetics Cell Proliferation / genetics Cytokinesis / genetics Female Humans Kidney Failure, Chronic / genetics pathology therapy Male Micronuclei, Chromosome-Defective / statistics & numerical data Middle Aged Mouth Mucosa / cytology pathology Mutagenicity Tests / methods Predictive Value of Tests Renal Dialysis / adverse effects methods Sensitivity and Specificity

来  源:   DOI:10.1016/j.mrgentox.2017.07.001   PDF(Sci-hub)

Abstract:
Patients with end-stage renal disease (ESRD) require hemodialysis. However, dialysis therapy may cause genomic damage due to increased oxidative stress. Non-invasive assessment of genotoxicity may be helpful for developing management strategies. We applied the buccal micronucleus cytome (BMCyt) assay to ESRD patients on dialysis. Patients (n=35, age 52±2 year) on dialysis therapy (20.9±0.8months) had low glomerular filtration rates (GFR=5.00±0.36ml/min/1.73m2); controls (n=21, age 51±2 year) were healthy adults with no known recent illnesses or exposures. Patients had significantly increased chromosome damage: clastogenic/aneugenic events (frequency of cells with MN), cellproliferation (basal cells), cytokinesis defects (binucleated cells), and celldeath (pyknotic cells); Repair Index was lower in the patient group. Receiver Operator Characteristic (ROC) curve analysis showed that cells with MN were the best predictor for discriminating between patients and controls. Other predictivebiomarkers were the frequencies of basal, binucleated,and pyknotic.
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