Abstract：
In a case of negative human-leopard (Panthera pardus) interaction, an animal was burnt alive in South Rajasthan, India. We identified the species and sex of the victim animal from the ash using forensic DNA analysis. We recovered three objects (half burnt clot, stone, and shrub twig) from the ash having suspected blood stains. We extracted DNA from these items and amplified a partial fragment of mitochondrial DNA cytochrome b and 12S RNA genes. The sequence generated from these amplicons suggested that the victim animal was a leopard. Furthermore, amplification of a fragment of SRY (224 bp) and ZFX/Y (442 bp) genes indicated that the blood clot was of a male leopard. Although attempts have been made to remove every possible evidence from the crime scene, the species and sex of the victim animal were determined from the challenging and invisible blood stains wrapped in the ash.
摘要：
在负面的人豹（Pantherapardus）相互作用的情况下，一只动物在南拉贾斯坦邦被活活烧死,印度。我们使用法医DNA分析从灰烬中确定了受害者动物的种类和性别。我们找到了三个物体(一半烧焦的凝块，石头,和灌木树枝）从灰烬中怀疑有血迹。我们从这些项目中提取了DNA，并扩增了线粒体DNA细胞色素b和12SRNA基因的部分片段。从这些扩增子产生的序列表明受害动物是豹子。此外，SRY（224bp）和ZFX/Y（442bp）基因片段的扩增表明血凝块是雄性豹子。尽管已经尝试从犯罪现场清除所有可能的证据，受害者动物的种类和性别是由包裹在灰烬中的挑战性和无形的血迹确定的。
