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Abstract：
Odorous molecules in earthen-ponds rainbow trout aquaculture farming in Germany were investigated with a special focus on musty-earthy off-odorants. To this aim, fish meat and skin were extracted using solvent-assisted flavour evaporation (SAFE) and were mildly concentrated; extracts were subsequently analysed by means of one- and two-dimensional high-resolution gas chromatography coupled with mass spectrometry and olfactometry (GC-MS/O and 2D-HRGC-MS/O). Aroma extract dilution analysis (AEDA) of the solvent extracts revealed the presence of 76 odorants of which 75 were successfully identified. Thereby, rotundone (black pepper) is described for the first time as an odour-active substance in fish. Moreover, a series of compounds is described for the first time in German aquaculture rainbow trout fish, including, amongst others, (E,Z,Z)-2,4,7-tridecatrienal, (E)-4,5-epoxy-(E)-2-decenal, 4-ethyloctanoic acid, 3-methylindole (skatole), d-limonene, and indole. The analytical findings were further compared to sensory evaluation of the samples, and previously obtained data on the respective aquacultural water.
摘要：
对德国土塘虹鳟鱼水产养殖中的有气味分子进行了研究，特别关注发霉的土质异味剂。为了这个目标,使用溶剂辅助风味蒸发（SAFE）提取鱼肉和鱼皮，并进行适度浓缩；随后通过一维和二维高分辨率气相色谱以及质谱和嗅觉测定法（GC-MS/O和2D-HRGC-MS/O）分析提取物。溶剂提取物的香气提取物稀释分析（AEDA）显示存在76种气味剂，其中75种被成功鉴定。因此，rotundone（黑胡椒）首次被描述为鱼类中的气味活性物质。此外，在德国水产养殖虹鳟鱼中首次描述了一系列化合物，包括,在其他人中，(E,Z,Z)-2,4,7-十三三氨基酸，(E)-4,5-环氧-(E)-2-癸酸，4-乙基辛酸,3-甲基吲哚（skatole），d-柠檬烯,还有吲哚.将分析结果进一步与样品的感官评价进行比较，和先前获得的有关各自水产养殖用水的数据。
