3-甲基吲哚(Skatole),由肠道微生物活性产生的色氨酸的降解产物,显著有助于气味的滋扰。它对动物福利的不利影响,人类健康,注意到环境污染。然而,尚不清楚肠道微生物群是否介导硒(Se)对粪臭素产生的影响,以及潜在的机制仍然难以捉摸。硒化葡萄糖(SeGlu)衍生物是一种新型的有机硒化合物。在这项研究中,不同范围的饮食SeGlu处理水平,包括SeGlu缺陷(CK),SeGlu-adequate(0.15mgSe/L),和SeGlu-超营养(每升0.4毫克硒)条件,研究了SeGlu对雄性Sprague-Dawley(SD)大鼠肠道微生物组和血清代谢组变化的复杂相互作用。研究表明,SeGlu的补充增强了大鼠的抗氧化能力,显着表现在过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)的活性增加,而丙二醛(MDA)水平无变化。宏基因组测序分析验证了SeGlu治疗组显着增加了有益微生物的丰度,例如梭菌,Ruminococus,粪杆菌,乳酸菌,和Alloprevotella,同时显着降低了机会性病原体的丰度,例如拟杆菌和Alistipes。进一步的代谢组学分析显示苯丙氨酸,酪氨酸,SeGlu处理组的色氨酸生物合成发生变化。值得注意的是,吲哚的生物合成,一条关键的道路,受到SeGlu治疗的影响,涉及几种关键酶。相关性分析表明特定细菌种类-密螺旋体,拟杆菌,和Ruminococus,以及吲哚和衍生物浓度的变化。此外,通过粪便微生物群移植证实了SeGlu处理的粪便微生物群的功效,导致大鼠粪便素浓度降低。总的来说,对不同SeGlu水平的微生物群和代谢组反应的分析表明,SeGlu是一种有前途的饮食添加剂,可调节肠道微生物群并减少家畜和家禽业的气味。
3-Methylindole (
Skatole), a degradation product of tryptophan produced by intestinal microbial activity, significantly contributes to odor nuisance. Its adverse effects on animal welfare, human health, and environmental pollution have been noted. However, it is still unclear whether the intestinal microbiota mediates the impact of selenium (Se) on
skatole production and what the underlying mechanisms remain elusive. A selenized glucose (SeGlu) derivative is a novel organic selenium compound. In this study, a diverse range of dietary SeGlu-treated levels, including SeGlu-deficient (CK), SeGlu-adequate (0.15 mg Se per L), and SeGlu-supranutritional (0.4 mg Se per L) conditions, were used to investigate the complex interaction of SeGlu on intestinal microbiome and serum metabolome changes in male Sprague-Dawley (SD) rats. The study showed that SeGlu supplementation enhanced the antioxidant ability in rats, significantly manifested in the increases of the activity of catalase (CAT) and glutathione peroxidase (GSH-Px), while no change in the level of malonaldehyde (MDA). Metagenomic sequencing analysis verified that the SeGlu treatment group significantly increased the abundance of beneficial microorganisms such as Clostridium, Ruminococcus, Faecalibacterium, Lactobacillus, and Alloprevotella while reducing the abundance of opportunistic pathogens such as Bacteroides and Alistipes significantly. Further metabolomic analysis revealed phenylalanine, tyrosine, and tryptophan biosynthesis changes in the SeGlu treatment group. Notably, the biosynthesis of indole, a critical pathway, was affected by SeGlu treatment, with several crucial enzymes implicated. Correlation analysis demonstrated strong associations between specific bacterial species - Treponema, Bacteroides, and Ruminococcus, and changes in indole and derivative concentrations. Moreover, the efficacy of SeGlu-treated fecal microbiota was confirmed through fecal microbiota transplantation, leading to a decrease in the concentration of
skatole in rats. Collectively, the analysis of microbiota and metabolome response to diverse SeGlu levels suggests that SeGlu is a promising dietary additive in modulating intestinal microbiota and reducing odor nuisance in the livestock and poultry industry.