Abstract：
Genetic information in genomes is ordered, arranged in such a way that it constitutes a code, the so-called cis regulatory code. The regulatory machinery of the cell, termed trans-factors, decodes and expresses this information. In this way, genomes maintain a potential repertoire of genetic programs, parts of which are executed depending on the presence of active regulators in each condition. These genetic programs, executed by the regulatory machinery, have functional units in the genome delimited by punctuation-like marks. In genetic terms, these informational phrases correspond to transcription units, which are the minimal genetic information expressed consistently from initiation to termination marks. Between the start and final punctuation marks, additional marks are present that are read by the transcriptional and translational machineries. In this work, we look at all the experimentally described and predicted genetic elements in the bacterium Escherichia coli K-12 MG1655 and define a comprehensive architectural organization of transcription units to reveal the natural genome-design and to guide the construction of synthetic genetic programs.
摘要：
基因组中的遗传信息是有序的，以构成代码的方式排列，所谓的顺式监管代码。细胞的监管机构，称为反式因子，解码并表达这些信息。这样,基因组保持着潜在的遗传程序，其中的部分根据在每种情况下有源调节器的存在而执行。这些基因程序，由监管机构执行，在基因组中具有由类似标点符号的标记界定的功能单元。在基因方面,这些信息短语对应于转录单位，它们是从起始标记到终止标记一致表达的最小遗传信息。在开始和最后的标点符号之间，存在由转录和翻译机制读取的额外标记。在这项工作中,我们研究了大肠杆菌K-12MG1655中所有实验描述和预测的遗传元件，并定义了转录单元的全面结构组织，以揭示自然基因组设计并指导合成遗传程序的构建。
