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Abstract：
Rhizome of Ligusticum chuanxiong is an effective medical plant, which has been extensively applied for centuries in migraine and cardiovascular diseases treatment in China. Polysaccharides from this plant have been shown to have interesting bioactivities, but previous studies have only been performed on the neutral polysaccharides. In this study, LCP-I-I, a pectic polysaccharide fraction, was obtained from the 100 °C water extracts of L. chuangxiong rhizomes and purified by diethylaminethyl (DEAE) sepharose anion exchange chromatography and gel filtration. Monosaccharide analysis and linkage determination in addition to Fourier transform infrared (FT-IR) spectrometer and Nuclear magnetic resonance (NMR) spectrum, indicated that LCP-I-I is a typical pectic polysaccharide, with homo-galacturonan and rhamnogalacturonan type I regions and arabinogalactan type I and type II (AG-I/AG-II) side chains. LCP-I-I exhibited potent complement fixation activity, ICH50 of 26.3 ± 2.2 µg/mL, and thus has potential as a natural immunomodulator.
摘要：
川芎根茎是一种有效的药用植物,在中国已经广泛应用了几个世纪的偏头痛和心血管疾病的治疗。来自该植物的多糖已被证明具有有趣的生物活性，但以前的研究只对中性多糖进行。在这项研究中,LCP-I-I,果胶多糖部分，从L.chuangxixong根茎的100°C水提取物中获得，并通过二乙基氨基乙基（DEAE）琼脂糖阴离子交换色谱和凝胶过滤进行纯化。单糖分析和连锁测定除了傅里叶变换红外(FT-IR)光谱仪和核磁共振(NMR)光谱,表明LCP-I-I是一种典型的果胶多糖，具有同型半乳糖醛酸和鼠李糖半乳糖醛酸I型区域以及阿拉伯半乳聚糖I型和II型（AG-I/AG-II）侧链。LCP-I-I表现出有效的补体固定活性，ICH50为26.3±2.2µg/mL，因此具有作为天然免疫调节剂的潜力。
